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Invitrogen
Human NAP-2/PPBP ELISA Kit
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Product Specifications
Analytical sensitivity
8.5 pg/mL
Assay range
8.5-1,000 pg/mL
Sample type/volume
Plasma
1 µL
Serum
1 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Protein name
PPBP
Protein family
Other Proteins
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human NAP-2/PPBP ELISA quantitates Hu NAP-2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NAP-2.
Principle of the method
The Human NAP-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Members of the a-chemokine subfamily of inducible, secreted, pro-inflammatory cytokines contain a similar motif, in which the first two cysteine residues are separated by a single residue (Cys-X-Cys), and are also chemotactic for neutrophils. The platelet basic protein (PBP), a member of the a-chemokine family, resides in the a-granules of platelets and is released upon their activation. Proteolytic cleavage of the amino terminus of PBP leads to the generation of several peptides, which include mature PBP, connective tissue-activating peptide III (CTAP III, also designated low affinity platelet factor IV (LA-PF4)), b-thromboglobulin (b-TG), and neutrophil-activating peptide 2 (NAP-2). PBP and its N-truncated derivatives mediate inflammation and wound healing. Specifically, NAP-2 activates chemotaxis and degranulation in neutrophils during inflammation. The gene encoding human PBP maps to chromosome 4q12-q13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, CXCL7, LA-PF4, LDGF, MDGF, NAP-2, PBP, PPBP, SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1
Gene ID : (Human) 5473
Gene symbol : PPBP
Protein Aliases : beta-thromboglobulin, C-X-C motif chemokine 7, chemokine (C-X-C motif) ligand 7, connective tissue-activating peptide III, CXC chemokine ligand 7, LDGF, Leukocyte-derived growth factor, low-affinity platelet factor IV, Macrophage-derived growth factor, MDGF, neutrophil-activating peptide 2, neutrophil-activating peptide-2, PBP, Platelet basic protein, pro-platelet basic protein (chemokine (C-X-C motif) ligand 7), small inducible cytokine B7, small inducible cytokine subfamily B, member 7, Small-inducible cytokine B7, thrombocidin 1, thrombocidin 2, thromboglobulin, beta-1
UniProt ID (Human) P02775
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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