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Invitrogen
Human NF-L ELISA Kit
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Human NF-L ELISA Kit
NF-L is readily detectable in CSF, Plasma and Serum. Endogenous levels were measured from multiple CSF (n=10), serum (n=10) and plasma (n=10) samples as depicted in scatter plots (median with interquartile range). Sample values may vary across different cohorts. For informational purposes only. (Medical histories of the donors were not provided.)
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Product Specifications
Analytical sensitivity
0.8 pg/mL
Assay range
1.6-1200
Sample type/volume
Cerebrospinal fluid
10 µL
Serum
50 µL
Plasma
50 µL
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 45 min
Homogenous (no wash)
No
Interassay CV
4%
Intraassay CV
3.7%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated microwell plate
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
NF-L
Species (tested)
Human, Mouse, Rat
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human NF-L ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of human NF-L in CSF, serum, and plasma (citrate and heparin) and cell culture supernatants (CCS).
Target information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CMT1F, CMT2E, CMTDIG, NF-L, NF68, NFL, PPP1R110
Gene ID : (Human) 4747, (Rat) 83613, (Mouse) 18039
Gene symbol : NEFL, Nefl, Nefl
Protein Aliases : 68 kDa neurofilament protein, 68kDa neurofilament, FLJ53642, NEF3, NEFL, neurofilament, neurofilament 3, neurofilament-l, neurofilament-light, N-FL, NFM, NF-M, NLC, OTTHUMP00000196614, intermediate filament, light molecular weight neurofilament protein, neurofilament protein, light chain, neurofilament subunit NF-L, neurofilament triplet L protein, light polypeptide, light polypeptide 68kDa, protein phosphatase 1, regulatory subunit 110, unnamed protein product
UniProt ID (Human) P07196, (Rat) P19527, (Mouse) P08551
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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