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Invitrogen
Human PMN (Neutrophil) Elastase ELISA Kit
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Product Specifications
Analytical sensitivity
0.1 ng/mL
Assay range
0.16-10.0 ng/mL
Sample type/volume
Cerebrospinal Fluid
10 µL
Lavage Fluid
10 µL
Plasma
10 µL
Supernatant
10 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 20 min
Homogenous (no wash)
No
Interassay CV
5.6%
Intraassay CV
4.8%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Neutrophil elastase
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human PMN (Neutrophil) Elastase) ELISA quantitates Hu PMN (Neutrophil) Elastase in human plasma, exudate, bronchoalveolar lavage fluid, cerebrospinal fluid and seminal plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant PMN (Neutrophil) Elastase.
Principle of the method
The Human PMN (Neutrophil) Elastase solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains (see glycosylation). It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : ELA2, ELANE, GE, HLE, HNE, NE, PMN-E, SCN1
Gene ID : (Human) 1991
Gene symbol : ELANE
Protein Aliases : Bone marrow serine protease, ELA2A, Elastase, Elastase2, PE-1, elastase 2, neutrophil, Elastase-2, granulocyte-derived elastase, HLE, Human leukocyte elastase, leukocyte elastase, Medullasin, Neutrophil elastase, PMN elastase, polymorphonuclear elastase
UniProt ID (Human) P08246
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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