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Invitrogen
Human PR3 Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
0.19 ng/mL
Assay range
0.31-20 ng/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
PR3
Species (tested)
Human
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Human PR3 (Proteinase 3) ELISA quantitates PR3 in serum and plasma.
Principle of the method
The Human Proteinase 3 (PR3) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Proteinase 3 (PR3) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Proteinase 3 (PR3). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Proteinase 3 (PR3) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) (PubMed:3198760, PubMed:2033050, PubMed:28240246). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration (PubMed:23202369). May play a role in neutrophil transendothelial migration, probably when associated with CD177 (PubMed:22266279). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : ACPA, AGP7, C-ANCA, CANCA, MBN, MBT, NP-4, NP4, P29, PR-3, PR3
Gene ID : (Human) 5657
Gene symbol : PRTN3
Protein Aliases : azurophil granule protein 7, C-ANCA antigen, leukocyte proteinase 3, neutrophil proteinase 4, serine proteinase, neutrophil, unnamed protein product, wegener autoantigen, Wegener granulomatosis autoantigen
UniProt ID (Human) P24158
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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