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Invitrogen
Human Prostaglandin E2 ELISA Kit
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Product Specifications
Assay range
31.3-4,000 pg/mL
Sample type/volume
Plasma
100 µL
Urine
100 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
11.7%
Intraassay CV
6%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Tris Buffer
Alkaline Phosphatase Tracer
Leukotriene B4 Antiserum
DEA Buffer
Wash Buffer
pNPP tablets
Adhesive Plate Covers
Standard
Tris Buffer
Alkaline Phosphatase Tracer
Leukotriene B4 Antiserum
DEA Buffer
Wash Buffer
pNPP tablets
Adhesive Plate Covers
Shipping conditions
Dry ice
Storage
Store kit at -20°C. Refer to product documentation for component specific storage temperature.
Protein name
PGE2
Species (tested)
Human
Assay kit format
Competitive ELISA
Label or dye
AP
About This Kit
The Human Prostaglandin E2 ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (ELISA). This assay is designed to detect and quantify the level of human prostaglandin E2 in biological samples.
Principle of the method
This assay is based on the competition between Prostaglandin E 2 (PGE2) and a PGE2 alkaline phosphatase tracer for a limited amount of PGE2 specific monoclonal antibody. Because of the competition between PGE2 in the sample and PGE2 tracer for the PGE2 antibody, the signal obtained with the assay will be inversely proportional to the amount of PGE 2 in each sample.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Prostaglandin E2 (PGE2) is a lipid mediator derived from arachidonic acid through the actions of cyclooxygenase (COX) enzymes and terminal prostaglandin E synthases (PGES). It plays a significant role in mediating inflammation, pain sensation, and fever by acting on four receptor subtypes: EP1, EP2, EP3, and EP4. PGE2's involvement in inflammatory responses is well-documented, particularly in diseases such as rheumatoid arthritis and osteoarthritis. Various medications, including nonsteroidal anti-inflammatory drugs (NSAIDs) and selective COX-2 inhibitors, are used to reduce PGE2 production, thereby alleviating inflammation and pain. However, these drugs can have side effects, such as gastrointestinal issues and increased risk of thrombosis. Additionally, PGE2 has a critical role in immune regulation, affecting T cell function and, by extension, the adaptive immune response. It also has a significant impact on skeletal disorders and pain through mechanisms that are still being explored.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases : C20H32O5, PGE2, Prostaglandin E2
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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