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Invitrogen
Human SEMA3A ELISA Kit
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Product Specifications
Analytical sensitivity
0.1 ng/mL
Assay range
0.16-10 ng/mL
Sample type/volume
Cell Lysate
100 µL
Plasma
100 µL
Serum
100 µL
Tissue Homogenate
100 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
SEMA3A
Species (tested)
Human
Species (reported)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Semaphorin 3A (SEMA3A) ELISA quantitates SEMA3A in serum, plasma and other biological fluids.
Principle of the method
The Human Semaphorin 3A (SEMA3A) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : coll-1, COLL1, HH16, Hsema-I, Hsema-III, SEMA1, SEMA3A, SEMAD, SEMAIII, SEMAL, SemD
Gene ID : (Human) 10371
Gene symbol : SEMA3A
Protein Aliases : collapsin 1, MGC133243, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A, Sema III, semaphorin D, Semaphorin III, semaphorin L, Semaphorin-3A
UniProt ID (Human) Q14563
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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