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Invitrogen
Human SPARC ELISA Kit
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Product Specifications
Analytical sensitivity
0.11 ng/mL
Assay range
0.11-31.25 ng/mL
Sample type/volume
Supernatant
100 µL
Plasma
100 µL
Serum
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
12%
Intraassay CV
10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated microwell plate
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
SPARC
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human SPARC ELISA Kit is an enzyme-linked immunosorbent assay for quantitative detection of human SPARC (Osteonectin) in serum, plasma, and cell culture supernatants.
Target information
SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : BM-40, OI17, ON, SPARC
Gene ID : (Human) 6678
Gene symbol : SPARC
Protein Aliases : Basement membrane protein 40, BM40, Cysteine rich protein, Secreted acidic cystein rich glycoprotein, Secreted protein acidic and rich in cysteine (SPARC), Basement-membrane protein 40, BM-40, ON, Osteonectin, Secreted protein acidic and rich in cysteine, secreted protein, acidic, cysteine-rich (osteonectin), SPARC
UniProt ID (Human) P09486
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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