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Invitrogen
Human TIMP-1 ELISA (For Lysates) Kit
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Product Specifications
Analytical sensitivity
40 pg/mL
Assay range
40-18,000 pg/mL
Sample type/volume
Cell Lysate
50 µL
Tissue Homogenate
50 µL
Supernatant
10 µL
Plasma
10 µL
Serum
10 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Protein name
TIMP1
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human TIMP-1 quantitates human TIMP-1 in cell lysate, tissue homogenate. The assay will exclusively recognize both natural and recombinant human TIMP-1.
Target information
TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that functions by forms one-to-one complexes with target metallproteinases, such as collagenases, and irreversible inactivates them by binding to their catalytic zinc cofactor. TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16. It also functions as a growth factor that regulates cell differenation, migration, and cell death. TIMP1 activates cellular signaling cascades via CD63 and ITGB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CLGI, EPA, EPO, HCI, TIMP, TIMP-1, TIMP1
Gene ID : (Human) 7076
Gene symbol : TIMP1
Protein Aliases : Collagenase inhibitor, EPA, erythroid potentiating activity, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, FLJ90373, Metalloproteinase inhibitor, MMP inhibitor, RP1-230G1.3, TIMP 1, Metalloproteinase inhibitor 1, TIMP-1, Tissue inhibitor of metalloproteinases 1
UniProt ID (Human) P01033
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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