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Invitrogen
Human TfR/Transferrin R/CD71 Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
0.23 ng/mL
Assay range
0.39-25 ng/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Transferrin Receptor (CD71)
Species (tested)
Human
Assay kit format
Rapid ELISA Kit
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Human TFR1 (Transferrin Receptor 1) ELISA quantitates TFR1 in serum and plasma.
Principle of the method
The Human Transferrin Receptor 1 (TFR1) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Transferrin Receptor 1 (TFR1) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Transferrin Receptor 1 (TFR1). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Transferrin Receptor 1 (TFR1) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CD71, IMD46, p90, T9, TFR, TFR1, TFRC, TR, TRFR
Gene ID : (Human) 7037
Gene symbol : TFRC
Protein Aliases : CD antigen CD71, CD71, p90, T9, TR, transferrin receptor, Transferrin receptor 1, Transferrin receptor protein 1, unnamed protein product
UniProt ID (Human) Q1HE24
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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