Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.11 ng/mL

Assay range

0.11-75 ng/mL

Sample type/volume

Serum
50 µL
Supernatant
50 µL
Plasma
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

Cardiac Troponin C

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human Troponin C quantitates human Troponin C in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Troponin C.

Target information

Troponin (Tn) is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments, and as a result of calcium-ion uptake by troponin-C, the elements of the muscle thin filament undergo a series of allosteric changes which allow the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : CMD1Z, CMH13, TN-C, TNC, TNNC, TNNC1

Gene ID : (Human) 7134

Gene symbol : TNNC1

Protein Aliases : cardiac troponin C, slow twitch skeletal/cardiac muscle troponin C, TN-C, troponin C type 1 (slow), Troponin C, slow skeletal and cardiac muscles, troponin C1, slow

UniProt ID (Human) P63316

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