Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.09 ng/mL

Assay range

0.16-10 ng/mL

Sample type/volume

Plasma
100 µL
Serum
100 µL

Hands-on time

1 hr 20 min

Time-to-result

3 hr 30 min

Homogenous (no wash)

No

Interassay CV

<10%

Intraassay CV

<10%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Micro ELISA Plate (Dismountable)
Reference Standard
Concentrated Biotinylated Detection Ab(100x)
Concentrated HRP Conjugate (100x)
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer(25x)
Substrate Reagent
Stop Solution
Plate Sealer
Product Description
Certificate of Analysis

Shipping conditions

Wet ice

Storage

Refer to product documentation for component specific storage temperature.

Protein name

ZO-1

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human ZO-1 ELISA Kit quantifies ZO-1 in serum and plasma.

The Human ZO-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances.

Rigorous validation:

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Target information

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : TJP1, ZO-1, ZO1

Gene ID : (Human) 7082

Gene symbol : TJP1

Protein Aliases : DKFZp686M05161, MGC133289, OTTHUMP00000174520, Tight junction protein 1, Tight junction protein ZO-1, zona occludens 1, Zona occludens protein 1, zonula occludens 1 protein, Zonula occludens protein 1

UniProt ID (Human) Q07157

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