The Human Soluble Interleukin 6 Receptor(Hu sIL-6R) ELISA quantitates Hu sIL-6R in human serum, plasma, cell culture supernatants, urine, amniotic fluid or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sIL-6R.
Principle of the method
The Human sICAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
IL6R (interleukin-6 receptor subunit alpha) is a part of the receptor for interleukin 6. The IL-6 receptor complex is composed of two membrane glycoproteins: the low affinity receptor and the signaltransducing component. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.