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Invitrogen
IP3 Competitive ELISA Kit
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Product Specifications
Analytical sensitivity
9.38 pg/mL
Assay range
15.63-1,000 pg/mL
Sample type/volume
Serum
100 µL
Plasma
100 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Micro ELISA Plate (Dismountable)
Reference Standard
Concentrated Biotinylated Detection Ab(100x)
Concentrated HRP Conjugate (100x)
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer(25x)
Substrate Reagent
Stop Solution
Plate Sealer
Product Description
Certificate of Analysis
Reference Standard
Concentrated Biotinylated Detection Ab(100x)
Concentrated HRP Conjugate (100x)
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer(25x)
Substrate Reagent
Stop Solution
Plate Sealer
Product Description
Certificate of Analysis
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
IP3
Species (tested)
Chemical
Assay kit format
Competitive ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
IP3 Competitive ELISA Kit quantifies IP3 in serum and plasma.
The IP3 Competitive ELISA research-use-only kit is designed to quantitatively measure IP3. A IP3 standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to IP3 is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of IP3 in the samples is then determined by comparing the OD of the samples to the standard curve..
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Inositol 1,4,5-trisphosphate (IP3) is a crucial intracellular signaling molecule that plays an essential role in various cellular processes by regulating calcium release from the endoplasmic reticulum. IP3 is generated through the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) by the enzyme phospholipase C (PLC) following receptor activation. The released IP3 binds to its receptors, inositol 1,4,5-trisphosphate receptors (ITPRs), which are located on the endoplasmic reticulum membrane. This binding triggers the release of Ca2+ ions into the cytoplasm, leading to numerous downstream effects, including muscle contraction, secretion, metabolism, and cell growth. ITPRs are encoded by different genes leading to multiple isoforms (ITPR1, ITPR2, and ITPR3), each showing distinct tissue distribution and regulatory properties. Understanding the specific functions and regulatory mechanisms of IP3 and ITPRs is critical for elucidating their roles in health and disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : Inositol Triphosphate, Inositol 1, 4, 5-trisphosphate
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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