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Product Specifications
Analytical sensitivity
0.21 ng/mL
Assay range
4.7-300 ng/mL
Sample type/volume
Plasma
10 µL
Serum
10 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
3%
Intraassay CV
5.6%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Storage
2-8°C
Protein name
Human IgG3
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Immunoglobulin G3 (Hu IgG3) ELISA quantitates Hu IgG3 in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu IgG3.
Principle of the method
The Human IgG3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Human IgG3, also known as human immunoglobulin G3, is a subclass of human IgG antibodies that is produced by the immune system in response to antigens. It is the least abundant subclass of IgG in the blood and plays a minor role in defense against pathogens. IgG3 works by binding to specific antigens and promoting their clearance from the body through various mechanisms, including phagocytosis and complement activation. IgG3 is also involved in other immune responses, such as antibody-dependent cellular cytotoxicity and neutralization of toxins. Human IgG3 can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious and autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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