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Product Specifications
Analytical sensitivity
0.17 µIU/mL
Assay range
5.1-250 µlU/mL
Sample type/volume
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
45 min
Homogenous (no wash)
No
Interassay CV
8.5%
Intraassay CV
5.4%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Incubation Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Controls
Shipping condition
Wet Ice
Storage
2-8°C
Protein name
Insulin
Protein aliases
Insulin, Insulin A chain, Insulin B chain, preproinsulin, proinsulin
Protein family
Hormones & Receptors
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
Gene aliases
IDDM, IDDM1, IDDM2, ILPR, INS, IRDN, MODY10
Gene ID
Gene symbol
INS
UniProt ID
About This Kit
The Human Insulin ELISA Kit quantifies human insulin in serum. The assay will recognize both the natural and recombinant insulin.
Principle of the method
The Human insulin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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