Invitrogen

Human PARP (Cleaved) [214/215] ELISA Kit

6 References

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Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support
Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

<0.062 ng/mL

Assay range

0.156-10 ng/mL

Sample type/volume

Cell Lysate
10 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr

Homogenous (no wash)

No

Interassay CV

5.3%

Intraassay CV

4.6%

Instrument

Absorbance Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

PARP1 (cleaved Asp214)

Target sub-specificity

Cleaved

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

The Human PARP (Cleaved) [214/215] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of PARP (Cleaved) [214/215] ) in fresh or frozen human cell lysates. The assay recognizes both natural and recombinant human PARP (Cleaved) [214/215].

Principle of the method

A monoclonal capture antibody specific for PARP (Cleaved) [214/215] has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Target information

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Gene aliases : ADPRT, ADPRT 1, ADPRT1, ARTD1, pADPRT-1, PARP, PARP-1, PARP1, PPOL

Gene ID : (Human) 142

Gene symbol : PARP1

Protein Aliases : ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase), ADP-ribosyltransferase diphtheria toxin-like 1, ADP-ribosyltransferase NAD(+), ADPRT 1, ARTD1, DNA ADP-ribosyltransferase PARP1, NAD(+) ADP-ribosyltransferase 1, PARP-1, poly (ADP-ribose) polymerase 1, poly (ADP-ribose) polymerase family, member 1, Poly [ADP-ribose] polymerase 1, poly(ADP-ribose) polymerase, poly(ADP-ribose) synthetase, poly(ADP-ribosyl)transferase, Poly[ADP-ribose] synthase 1, Protein poly-ADP-ribosyltransferase PARP1

UniProt ID (Human) P09874

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If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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