C-C motif chemokine 2, immediate-early serum-responsive JE protein, Immediate-early serum-responsive protein JE, MCP-1, Monocyte chemoattractant protein 1, monocyte chemoattractant protein-1, Monocyte chemotactic protein 1, small inducible cytokine A2, small inducible gene JE, Small-inducible cytokine A2
The Rat Monocyte Chemoattractant Protein 1 (MCP-1) ELISA quantitates Rt MCP-1 in rat serum, plasma, cell culture supernatants,amniotic fluid or other body fluids. The assay will exclusively recognize both natural and recombinant Rt MCP-1.
Principle of the method
The Rat MCP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.