Analytical sensitivity
0.8 pg/mL
Assay range
0.8-50 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Hands-on time
1 hr 45 min
Time-to-result
24 hr 30 min
Homogenous (no wash)
No
Instrument
Colorimetric Microplate Reader
Product size
10 x 96 tests
Contents
Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer: Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme
Enzyme: Pre-titrated Avidin-HRP
Substrate: 1X TMB Solution
Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards
96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Storage
2-8°C
Protein name
CCL3 (MIP-1 alpha)
Protein aliases
C-C motif chemokine 3, Heparin-binding chemotaxis protein, L2G25B, Macrophage inflammatory protein 1-alpha, macrophage inflammatory protein-1alpha, MIP-1 alpha, MIP-1-alpha, MIP1 (a), SIS-alpha, small inducible cytokine A3, Small-inducible cytokine A3, TY-5
Species (tested)
Mouse
Assay kit format
Uncoated ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
Gene aliases
AI323804, Ccl3, G0S19-1, LD78alpha, MIP-1alpha, MIP1-(a), MIP1-alpha, Mip1a, Scya3
Gene ID
Gene symbol
Ccl3
UniProt ID
The Mouse Macrophage Inflammatory Protein 1 alpha (CCL3) (MIP-1α) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse MIP-1α. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Macrophage inflammatory protein-1a (MIP-1a) is an 8kDa member of the C-C chemokine subfamily. C-C chemokines are chemoattractants and activators for monocytes and T cells. MIP-1a has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1b and RANTES.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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