Capture Antibody: Pre-titrated, purified antibody Detection Antibody: Pre-titrated, biotin-conjugated antibody Standard: Recombinant protein for generating standard curve and calibrating samples 10X Coating Buffer: Buffer for plating the Capture Antibody 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme Enzyme: Pre-titrated Avidin-HRP Substrate: 1X TMB Solution Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22, -76, -86)
The Human Macrophage Inflammatory Protein 3 beta (CCL19) (MIP-3β) Uncoated ELISA Kit contains pre-matched antibody pairs ,plates, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human MIP-3β). Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
CCL19, also known as MIP-3 beta (Macrophage Inflammatory Protein 3 beta), is a member of the CC- subfamily of chemokines. It is most closely related to CCL21, with which it shares 32% amino acid sequence homology. CCL19 and CCL21 are expressed mainly by stromal cells in the T cell-rich zones of lymph nodes. They are critical mediators of the homeostatic trafficking of naive T cells and activated dendritic cells into the secondary lymphoid organs. CCL19 and CCL21 also play a role in T cell priming and activation, as well as the recruitment of lymphocytes to inflamed tissue. Both proteins signal via the G protein-coupled receptor, CCR7, which is expressed on T cells and mature dendritic cells. Although CCL19 and CCL21 both exhibit the same affinity for CCR7, only the binding of CCL19 results in the desensitization and internalization of the receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.