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Invitrogen
Mouse B7-H3 (CD276) ELISA Kit
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Product Specifications
Analytical sensitivity
0.21 ng/mL
Assay range
0.21-320 ng/mL
Sample type/volume
Serum
100 µL
Plasma
100 µL
Supernatant
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
12%
Intraassay CV
10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated microwell plate
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
B7-H3 (CD276)
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Mouse B7-H3 (CD276) ELISA Kit is an enzyme-linked immunosorbent assay for quantitative detection of mouse B7-H3 in serum, plasma, and cell culture supernatants.
Target information
CD276 is a member of the B7 family of co-stimulatory molecules also known as B7-H3. CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells and selectively stimulates the production of interferon gamma. Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis and colon. In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing. CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : 6030411F23Rik, AU016588, B7h3, B7RP-2, Cd276
Gene ID : (Mouse) 102657
Gene symbol : Cd276
Protein Aliases : B7 homolog 3, B7-H3, CD276, CD276 antigen, Costimulatory molecule, sB7 H3, sB7H3, sCD276, soluble B7 H3, soluble B7H3, soluble CD276
UniProt ID (Mouse) Q8VE98
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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