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Invitrogen
Mouse Basic IL-10 ELISA Kit
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Product Specifications
Analytical sensitivity
5 pg/mL
Assay range
15.6-1,000 pg/mL
Sample type/volume
Serum
50 µL
Supernatant
50 µL
Plasma
50 µL
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Coated Microwell Strips
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
IL-10
Protein family
Hormones & Receptors
Species (tested)
Mouse
Species (reported)
Mouse
Assay kit format
Basic ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The mouse Interleukin-10 (IL-10) ELISA kit is designed for the quantitative measurement of mouse IL 10 in a range of biological samples, including murine serum, plasma, buffered solutions, or cell culture medium. This assay has high specificity and can accurately detect both natural and recombinant forms of mouse IL-10.
Principle of the method
The mouse IL-10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) employs a well-matched pair of antibodies to capture and quantify the target analyte. The microplate wells are coated with a specific antibody that selectively binds to Hu IL-10. Subsequently, the samples, standards, or controls are added to the wells, allowing the target to bind to the immobilized (capture) antibody. The addition of a second (detector) antibody forms a sandwich complex. A substrate solution is then introduced, initiating a reaction with the enzyme-antibody-target complex, leading to the generation of a measurable signal. The intensity of this signal is directly proportional to the concentration of Hu IL-10 present in the original specimen.
Target information
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CSIF, Il-10, Il10
Gene ID : (Mouse) 16153
Gene symbol : Il10
Protein Aliases : CSIF, Cytokine synthesis inhibitory factor, IL-10, H-IL-10, il 10, ILN, Interleukin, Interleukin10, MGC126450, MGC126451, RP11-262N9.1, Interleukin-10
UniProt ID (Mouse) P18893
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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