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Invitrogen
Mouse Basic IL-12 ELISA Kit
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Product Specifications
Analytical sensitivity
4 pg/mL
Assay range
31.3-2,000 pg/mL
Sample type/volume
Serum
50 µL
Supernatant
50 µL
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Coated Microwell Strips
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
IL-12 p70
Protein family
Hormones & Receptors
Species (tested)
Mouse
Assay kit format
Basic ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Mouse Interleukin-12 (Ms IL-12) ELISA kit enables the precise quantification of in mouse serum or cell culture medium. This highly specific assay is designed to exclusively detect both endogenous and recombinant forms of Ms IL-12.
Principle of the method
The Mouse IL-12 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) employs a meticulously paired set of antibodies to capture and measure the target analyte. The wells of the microplate are pre-coated with a target-specific antibody, facilitating the binding of the analyte during the assay. Following the addition of samples, standards to the wells, they bind to the immobilized (capture) antibody. The formation of a sandwich complex is achieved by introducing a second (detector) antibody. Subsequently, a substrate solution is added, initiating a reaction with the enzyme-antibody-target complex and generating a measurable signal. The intensity of this signal is directly proportional to the concentration of the target present in the original specimen.
Target information
IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : Il-12a, Il-12b, IL-12p35, Il-12p40, Il12a, Il12b, Il12p40, Ll12a, p35, p40
Gene ID : (Mouse) 16159, (Mouse) 16160
Gene symbol : Il12a, Il12b
Protein Aliases : CLMF p35, CLMF p40, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p35, IL-12 subunit p40, IL-12A, IL-12B, 20C2, il 12, IL 12p70, IL-12 p75, IL12A/B, IL12AB, ILN, Interleukin, Interleukin-12 p70, MGC151228, MGC151232, Interleukin-12 subunit alpha, Interleukin-12 subunit beta
UniProt ID (Mouse) P43431, (Mouse) P43432
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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