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Invitrogen
Mouse Basic IL-2 ELISA Kit
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Product Specifications
Analytical sensitivity
5.3 pg/mL
Assay range
15.6-1,000 pg/mL
Sample type/volume
Serum
50 µL
Supernatant
50 µL
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Coated Microwell Strips
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
IL-2
Protein family
Hormones & Receptors
Species (tested)
Mouse
Species (reported)
Mouse
Assay kit format
Basic ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The mouse Interleukin-2 (IL-2) ELISA kit facilitates the accurate quantification of mouse IL-2 in various biological samples, including murine serum, plasma, or cell culture medium. This highly specific assay is designed to exclusively detect both endogenous and recombinant forms of mouse IL-2.
Principle of the method
The mouse IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) employs a meticulously paired set of antibodies to capture and measure the target analyte. The microplate wells are pre-coated with a target-specific antibody that allows for the binding of the analyte during the assay. Upon the addition of samples or standards to the wells, they specifically bind to the immobilized (capture) antibody. The formation of a sandwich complex is achieved by introducing a second (detector) antibody. Subsequently, a substrate solution is added, initiating a reaction with the enzyme-antibody-target complex and generating a measurable signal. The intensity of this signal is directly proportional to the concentration of the target present in the original specimen.
Target information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : Il-2, Il2
Gene ID : (Mouse) 16183
Gene symbol : Il2
Protein Aliases : IL-2, T-cell growth factor, TCGF, H-IL-2, il 2, ILN, Interleukin, Interleukin2, Involved in regulation of T cell clonal expansion, M-IL-2, T-cell growth factor (TCGF), Interleukin-2
UniProt ID (Mouse) P04351
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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