Search
Invitrogen
Mouse Basic TNF alpha ELISA Kit
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Product Specifications
Analytical sensitivity
3.7 pg/mL
Assay range
31.3-2,000 pg/mL
Sample type/volume
Serum
50 µL
Supernatant
50 µL
Plasma
50 µL
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Coated Microwell Strips
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
TNF alpha
Protein family
Hormones & Receptors
Species (tested)
Mouse
Species (reported)
Mouse
Assay kit format
Basic ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Mouse Tumor Necrosis Factor Alpha (Mu TNF alpha) ELISA kit offers a precise method for quantifying Mu TNF alpha in murine serum, plasma, urine, or cell culture medium. The test will exclusively identify both natural and genetically engineered Mu TNF alpha.
Principle of the method
The solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for mouse TNF alpha is engineered to quantify the amount of the target captured between a compatible antibody pair. An antibody specific to the target has been pre-coated in the wells of the provided microplate. Samples, standards, or controls are subsequently introduced into these wells and attach to the immobilized (capture) antibody. The sandwich is completed by the addition of the second (detector) antibody. A substrate solution is then added, which reacts with the enzyme-antibody-target complex to yield a measurable signal. The strength of this signal is directly related to the concentration of the target in the original sample.
Target information
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Gene aliases : DIF, Tnf, TNF-a, TNF-alpha, Tnfa, TNFalpha, Tnfsf1a, TNFSF2
Gene ID : (Mouse) 21926
Gene symbol : Tnf
Protein Aliases : Cachectin, DADB-70P7.1, TNF alpha, TNF superfamily, TNF α, TNFα, TNF-a, TNF-alpha, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
UniProt ID (Mouse) P06804
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support