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Invitrogen
Mouse CD48 Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
9.38 pg/mL
Assay range
15.63-1000 pg/mL
Sample type/volume
Serum
50 µL
Plasma
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
CD48
Species (tested)
Mouse
Detector antibody conjugate
HRP
Label or dye
Unconjugated
About This Kit
The Mouse CD48 (Cluster Of Differentiation 48) ELISA quantitates CD48 in serum and plasma.
Principle of the method
The Mouse Cluster Of Differentiation 48 (CD48) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the concentration of Cluster Of Differentiation 48 (CD48) in biological samples. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are added into these wells and bind to the immobilized (capture) antibody.
The sandwich complex is formed by the addition of a second (HRP-linked) detection antibody specific to Cluster Of Differentiation 48 (CD48). Excess reagents are washed from the plate.
A substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal. The enzyme-substrate reaction is terminated by the addition of stop solution. The intensity of the signal, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Cluster Of Differentiation 48 (CD48) present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
CD48, also known as Blast-1, is a 45 kDa glycoprotein that is part of the SLAM and Ig superfamilies. It is a GPI-anchored protein broadly expressed on most hematopoietic cells, including lymphocytes and monocytes, but is weakly expressed on granulocytes and absent on platelets, fibroblasts, epithelium, and endothelium. CD48 plays a critical role in adhesion and T cell activation, serving as a high-affinity ligand for 2B4 and a low-affinity ligand for CD2. The interaction between 2B4 and CD48 among NK and NK-T cells regulates cell proliferation. CD48 is involved in eosinophil activation and its expression increases in several infectious diseases. It is also a marker for detecting defects in the GPI anchoring structure in patients with paroxysmal nocturnal hemoglobulinuria (PNH). Recent studies have highlighted the differential expression of SLAM family members, including CD48, CD150, and CD244, among bone marrow hematopoietic progenitors. This provides a useful tool for predicting the primitiveness of hematopoietic progenitors: hematopoietic stem cells (HSCs) are CD150(+)CD244(-)CD48(-), non-self-renewing multipotent progenitors (MPPs) are CD244(+)CD150(-)CD48(-), and the most restricted progenitors are CD48(+)CD244(+)CD150(-). Additionally, the 2B4-CD48 interaction may be involved in the pathology of allergic airway diseases, offering a potential pharmacological target for treating conditions like allergic rhinitis and asthma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : Bcm-1, BCM1, BLAST, BLAST-1, BLAST1, MEM-102, Sgp-60, SLAMF2
Gene ID : (Mouse) 12506
Gene symbol : Cd48
Protein Aliases : B-cell activation marker, CD48 antigen (B cell membrane protein), CD48d, sCD 48, sCD48, Signaling lymphocytic activation molecule 2 (SLAMF2), soluble CD 48, soluble CD48, BCM1 surface antigen, HM48-1, MRC OX-45 surface antigen, signaling lymphocytic activation molecule 2, SLAM family member 2, unnamed protein product
UniProt ID (Mouse) P18181
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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