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Invitrogen
Mouse GAPDH ELISA Kit
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Product Specifications
Analytical sensitivity
1.66 ng/mL
Assay range
1.66-500 ng/mL
Sample type/volume
Supernatant
100 µL
Plasma
100 µL
Serum
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
12%
Intraassay CV
10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated microwell plate
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
GAPDH
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Mouse GAPDH ELISA Kit is an enzyme-linked immunosorbent assay for quantitative detection of mouse GAPDH in cell culture supernatants, plasma, and serum samples.
Target information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : Gapd, Gapd-s, Gapdh, Gapdhs, Gapds
Gene ID : (Mouse) 14433, (Mouse) 14447
Gene symbol : Gapdh, Gapdhs
Protein Aliases : EC 1.2.1.12, HGNC:4141, MGC88685, GAPDH, GAPDH-2, Glyceraldehyde-3-phosphate dehydrogenase, testis-specific, Peptidyl-cysteine S-nitrosylase GAPDH, Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2, Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
UniProt ID (Mouse) P16858, (Mouse) Q64467
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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