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Invitrogen
Mouse LIGHT ELISA Kit
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Product Specifications
Analytical sensitivity
0.21 ng/mL
Assay range
0.21-60 ng/mL
Sample type/volume
Supernatant
100 µL
Plasma
100 µL
Serum
100 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
12%
Intraassay CV
10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated microwell plate
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Standard
Biotinylated Detection Antibody
Streptavidin-HRP
Sample Diluent
Wash Buffer Concentrate
Assay Buffer concentrate
Stop Solution
Substrate Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
LIGHT
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Mouse LIGHT ELISA Kit is an enzyme-linked immunosorbent assay for quantitative detection of mouse LIGHT (TNFSF14) in serum, plasma, and cell culture supernatants.
Target information
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF (tumor necrosis factor) and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 (tumor necrosis factor receptor 1) and Fas. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C. elegans death receptor-3) protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (death associated protein 6). Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : HVEM-L, HVEML, LIGHT, LTg, Ly113, Tnfsf14
Gene ID : (Mouse) 50930
Gene symbol : Tnfsf14
Protein Aliases : CD258, sCD258, sLIGHT, soluble CD258, soluble LIGHT, TR2, Tumor necrosis factor ligand superfamily member 14
UniProt ID (Mouse) Q9QYH9
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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