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![Mouse Tau (Phospho) [pS199] ELISA Kit](https://www.thermofisher.com/antibody/images/650/KMB7041_SC_080327.jpg?time=20220826 "Mouse Tau (Phospho) [pS199] ELISA Kit")
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Mouse Tau (Phospho) [pS199] ELISA Kit
Natural Mouse Tau[pS199] extracted from mouse Neuro 2a cells was serially diluted in Standard Diluent Buffer. The optical density of each dilution was plotted against the standard curve. Parallelism between the natural and recombinant protein is demonstrated in Figure 1 and indicates that the standard accurately reflects natural Mouse Tau[pS199] content in samples.
![Mouse Tau (Phospho) [pS199] ELISA Kit](https://www.thermofisher.com/antibody/images/150/KMB7041_SC_080327.jpg?time=20220826)
![Mouse Tau (Phospho) [pS199] ELISA Kit](https://www.thermofisher.com/antibody/images/150/KMB7011_Curve_080130.jpg?time=20220826)
![Mouse Tau (Phospho) [pS199] ELISA Kit (KMB7041)](https://www.thermofisher.com/antibody/images/150/Sandwich-Direct-ELISA-Kit-Packaging-650x600.jpg?time=20220826)
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Product Specifications
Analytical sensitivity
<58.5 pg/mL
Assay range
313-20,000 pg/mL
Sample type/volume
Brain Homogenate
10 µL
Cell Lysate
5 µL
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr
Homogenous (no wash)
No
Interassay CV
3.8%
Intraassay CV
5.6%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Standard Dilution Buffer
Biotinylated Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Tau
Target site-specificity
Phosphorylated Ser199
Species (tested)
Mouse
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Mouse Tau (Phospho) [pS199] ELISA quantitates Ms Tau (Phospho) [pS199] in mouse cell lysates, cell culture medium and brand homogenates. The assay will exclusively recognize both natural and recombinant Ms Tau (Phospho) [pS199].
Principle of the method
The Ms Tau (Phospho) [pS199] solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : AI413597, AW045860, Mapt, Mtapt, Tau
Gene ID : (Mouse) 17762
Gene symbol : Mapt
Protein Aliases : Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, FLJ31424, FTDP17, Tau-4, Tau5, map tau, MGC138549, microtubules, neurofibrillary tangles, Neuronal Marker, PHFtau
UniProt ID (Mouse) P10637
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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