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Invitrogen
Multispecies AKT (Total/Phospho) InstantOne™ ELISA Kit
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_web-data.jpg?time=20220826 "Multispecies AKT (Total/Phospho) InstantOne™ ELISA Kit")
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Multispecies AKT (Total/Phospho) InstantOne™ ELISA Kit
Representative data for AKT (Total/Phospho) Multispecies InstantOne™ ELISA. MCF7 cells were pre-treated with 10 µM wortmannin washed and unstimulated (-) or stimulated with 10 ng/mL insulin for 10 min (+). Cells were analyzed for total AKT 1 (left) or phosphorylated AKT 1/2/3 (Ser473) (right) using the AKT (Total/Phospho) InstantOne™ ELISA.
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Product Specifications
Sample type/volume
Cell Lysate
20 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Capture Antibody
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Detection Antibody Reagent
InstantOne™ Assay Plate
Cell Lysis Buffer
Enhancer Solution
Wash Buffer (10X)
Substrate Reagent (TMB)
Stop Solution
Positive Control Cell Lysate (lyophilized)
Plate seal
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
AKT Pan
Target sub-specificity
Total
Species (tested)
Human
Assay kit format
InstantOne ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The InstantOne™ ELISA is specifically engineered for accurate measurement of human, mouse, and hamster total AKT 1 and phosphorylated AKT 1/2/3 (Ser473) in cell lysates. Detection of rat AKT 1 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Target information
AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : AKT, AKT1, AKT2, AKT3, CWS6, HIHGHH, MPPH, MPPH2, PKB, PKB-ALPHA, PKB-GAMMA, PKBB, PKBBETA, PKBG, PRKBA, PRKBB, PRKBG, RAC, RAC-ALPHA, RAC-BETA, RAC-gamma, RAC-PK-gamma, STK-2
Gene ID : (Human) 207, (Human) 208, (Human) 10000
Gene symbol : AKT1, AKT2, AKT3
Protein Aliases : AKT1m, DKFZp434N0250, kinase Akt1, MGC99656, pan akt, murine thymoma viral (v-akt) homolog-2, PKB, PKB alpha, PKB beta, PKB gamma, Protein kinase Akt-2, Protein kinase Akt-3, Protein kinase B, Protein kinase B alpha, Protein kinase B beta, Protein kinase B gamma, Proto-oncogene c-Akt, putative v-akt murine thymoma viral oncoprotein 2, rac protein kinase alpha, RAC protein kinase beta, RAC-alpha serine/threonine-protein kinase, RAC-beta serine/threonine-protein kinase, RAC-gamma serine/threonine protein kinase, RAC-gamma serine/threonine-protein kinase, RAC-PK-alpha, RAC-PK-beta, RAC-PK-gamma, serine-threonine protein kinase, STK-2, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene homolog 2, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), v-akt murine thymoma viral oncogene-like protein 1
UniProt ID (Human) P31749, (Human) P31751, (Human) Q9Y243
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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