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Invitrogen
Thyroxine (T4) Competitive ELISA Kit
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Product Specifications
Analytical sensitivity
0.33 ng/mL
Assay range
0.625-20 ng/mL
Sample type/volume
Fecal Extract
10 µL
Plasma
1 µL
Serum
1 µL
Supernatant
10 µL
Urine
2.5 µL
Hands-on time
1 hr 20 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
7%
Intraassay CV
3%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
-20°C
Protein name
Thyroxine (T4)
Species (tested)
Chemical
Assay kit format
Competitive ELISA
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Thyroxine (T4) ELISA quantitates Thyroxine (T4) in serum, plasma, urine, dried fecal extracts, or cell culture medium.
Principle of the method
The Thyroxine (T4) Competitive ELISA research-use-only kit is designed to quantitatively measure T4 present in serum, plasma, urine, extracted dried fecal samples, and tissue culture media samples. The kit offers two standard curve ranges. For serum and plasma samples we recommend using 10 µL of standards or samples. The assay concentration range for T4 will be from 20 ng/mL to 0.625 ng/mL. For urine samples we recommend using 100 µL of standards or samples. Assay concentrations of T4 that range from 4 ng/mL to 0.0625 ng/mL can be measured. A T4 stock solution is provided to generate standard curves for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A T4-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to T4 to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound T4-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Thyroxine is the main hormone produced by the thyroid gland. The thyroid hormones, triiodothyronine (T3) and thyroxine (T4), are tyrosine-based hormones produced by the thyroid gland that are primarily responsible for regulation of metabolism. Iodine is necessary for the production of T3 and T4. The major form of thyroid hormone in the blood is thyroxine, which has a longer half-life than T3. T4 is converted to the active T3 within cells by deiodinases (5'-iodinase). These are further processed by decarboxylation and deiodination to produce iodothyronamine and thyronamine. Thyroid hormone production is regulated via pituitary thyrotropin (TSH) modulation of thyroxine prohormone secretion by the thyroid gland and regulation of active triiodothyronine production. Thyroxine is identical across all species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases : C15H11I4NO4
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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