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Invitrogen
Triiodothyronine (T3) Competitive ELISA Kit
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Product Specifications
Analytical sensitivity
37.4 pg/mL
Assay range
78.125-5,000 pg/mL
Sample type/volume
Fecal Extract
100 µL
Plasma
100 µL
Serum
100 µL
Supernatant
100 µL
Urine
25 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
13.4%
Intraassay CV
6.3%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
HRP-Conjugated Detection Antibody
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Dry ice
Storage
-20°C
Protein name
Triiodothyronine (T3)
Species (tested)
Chemical
Assay kit format
Competitive ELISA
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Triiodothyronine (T3) ELISA quantitates T3 in serum, plasma, urine, dried fecal extracts, or cell culture medium.
Principle of the method
The Triiodothyronine (T3) Competitive ELISA research-use-only kit is designed to quantitatively measure T3 present in extracted serum and plasma, urine, extracted dried fecal samples, and tissue culture media samples. This kit measures total T3 in extracted serum and plasma and in extracted fecal samples. A T3 stock solution is provided to generate standard curves for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A T3-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a sheep antibody to T3 to each well. After a two-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound T3-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Thyroid hormones regulate a number of developmental, metabolic, and neural activities throughout the body. The thyroid gland synthesizes 2 hormones: Thyroxine, which contains 4 atoms of iodine (T4), and triiodothyronine (T3), which has 3 atoms of iodine. T3 production in the thyroid gland constitutes approximately 20% of the total T3; the rest is generated by the conversion (deiodination) of T4 to T3 in peripheral tissues. Thyroid hormones circulate primarily bound to carrier proteins (e.g., thyroidbinding globulin [TBG], prealbumin and albumin); whereas only a small fraction circulates unbound. The free form of T3 is the biologically active fraction. While both T3 and T4 are bound to TBG, T3 is bound less firmly than T4. Total T3 consists of both the bound and unbound fractions. Triiodothyronine (T3) is identical across all species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases : C15H12I3NO4
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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