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Invitrogen
Vitamin D3 Competitive ELISA Kit
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Product Specifications
Analytical sensitivity
0.94 ng/mL
Assay range
1.56-100 ng/mL
Sample type/volume
Cell Lysate
50 µL
Plasma
50 µL
Serum
50 µL
Tissue Homogenate
50 µL
Hands-on time
1 hr 20 min
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Vitamin D3
Species (tested)
All
Species (reported)
All
Assay kit format
Competitive ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Vitamin D3 (VD3) ELISA quantitates VD3 in serum, plasma and other biological fluids.
Principle of the method
The competitive multispecies ELISA research kit is designed to quantitatively measure Vitamin D3, independent of species . A Vitamin D3 standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Vitamin D3 is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. A TMB substrate solution is then added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Vitamin D3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Vitamin D3, also known as cholecalciferol, is a form of vitamin D that is synthesized in the skin upon exposure to ultraviolet B (UVB) light from the sun. It is the most biologically active form of vitamin D and plays a crucial role in maintaining overall health. Structurally, vitamin D3 is a steroid hormone precursor that undergoes metabolic processes in the liver and kidneys to be converted into its active form, known as calcitriol. Calcitriol acts as a hormone that regulates calcium and phosphorus metabolism in the body. The main function of vitamin D3 is to facilitate the absorption of calcium and phosphorus from the intestine, promoting their utilization for bone health and growth. It aids in the regulation of bone mineralization, ensuring the proper formation and maintenance of strong and healthy bones. Vitamin D3 is also involved in various other physiological processes beyond bone health. It plays a vital role in modulating immune function, helping to regulate the immune response and reduce the risk of autoimmune diseases. Additionally, it has been linked to cardiovascular health, muscle function, and mood regulation. In terms of localization, vitamin D receptors are present in various tissues throughout the body, including the intestines, bones, kidneys, immune cells, and brain. This widespread distribution of vitamin D receptors highlights the importance of maintaining adequate vitamin D3 levels for optimal functioning of these tissues and overall health.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases : Cholecalciferol
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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