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Accessible, high-performance cryo-EM at 200 kV
The Thermo Scientific Glacios 3 Cryo-TEM is an advanced platform for 200 kV cryo-EM imaging, expanding the capabilities and performance of the established Glacios platform. This latest system brings optical innovations to improve productivity and usability.
The Glacios 3 Cryo-TEM is equipped with the Thermo Scientific READY System, a built-in series of components that dramatically reduce infrastructure demands and facilitate installation. This integrated protection system would have eliminated the need for facility renovations at 80–95% of sites previously surveyed by Thermo Fisher Scientific for electron microscopy installations over the last decade.
With its new, compact footprint and integrated READY System, the Glacios 3 Cryo-TEM simplifies installation and infrastructure needs, enabling more organizations to bring cryo-EM in-house, integrating it earlier in the discovery and development pipelines.
Easier installation with the READY System brings cryo-EM to more lab spaces
Frequently asked questions about the Thermo Scientific Glacios 3 Cryo-TEM
High accuracy and improved throughput with aberration-free image shift
AFIS (aberration-free image shift) is a standard optical mode in Thermo Scientific cryo-TEMs that speeds up data collection without sacrificing image quality. Traditionally, the sample stage moves to align each foil hole with the electron beam, which slows down imaging, as the stage requires up to 30 seconds to relax after each movement. With AFIS, the beam is instead moved between foil holes while automatically correcting for astigmatism and coma.
Further improved with the Glacios 3 Cryo-TEM, AFIS now provides enhanced targeting accuracy, ultimately accelerating data acquisition with higher-quality imaging across the grid. The result is faster imaging with greater accuracy and less data required per experiment for high-resolution reconstruction, thereby reducing data storage demands.
Automated beam-centering improves on-target fringe-free imaging
Fresnel fringes create artifacts during cryo-EM imaging; fringe-free imaging (FFI) is an optical mode that minimizes these artifacts while allowing for a narrow electron beam to be used. This means more particles can be acquired per grid for higher throughput with single particle analysis. In cryo-ET, this allows more regions per lamella to be captured for better cellular coverage.
New to the Glacios 3 Cryo-TEM, an automated beam-centering routine for FFI helps ensure that the beam remains precisely aligned at the center of the field of view. This supports long-term, on-target data acquisition, increasing the amount of usable information captured in every image. As a result, fewer total images are needed to achieve high-resolution reconstructions, reducing overall data collection requirements per experiment, and consequently lowering long-term storage demands.
Easier cryo-TEM set-up with new optical model
During cryo-TEM experimental set-up, configuration of illumination parameters requires in-depth knowledge of electron optics. The 2-condenser lens system of a cryo-TEM makes the parallel beam illumination have discrete beam diameters and steps in the flux, which increase non-linearly (exponentially). Even for expert users, multiple tries are often needed to optimize the system configuration, and the ideal flux is difficult to achieve.
A new optical model in the Glacios 3 Cryo-TEM takes the guesswork out of cryo-EM experimental set-up. In a single click, it calculates all the lens current required along the optical path and sets the electron beam in parallel with the desired flux. As a result, set-up is now faster and easier, particularly for less experienced users. This also shortens the learning curve for new users.
Glacios Cryo-TEM webinar
For Research Use Only. Not for use in diagnostic procedures.