Technical Resources

Medium 254

A sterile, liquid medium prepared with 200 µM calcium chloride for the culture of human epidermal melanocytes. This basal medium requires the addition of HMGS (S-002-5) or HMGS-2 (S-016-5) prior to use. Below is the composition list. The concentrations are proprietary.

Catalog Number(s)
M254500

Components	Molecular Weight	Concentration (mg/L)	mM
Amino Acids
Glycine	75.0	confidential	n/a
L-Alanine	89.0	confidential	n/a
L-Alanyl-L-Glutamine	217.0	confidential	n/a
L-Arginine hydrochloride	211.0	confidential	n/a
L-Asparagine-H2O	150.0	confidential	n/a
L-Aspartic acid	133.0	confidential	n/a
L-Cysteine	121.0	confidential	n/a
L-Glutamic Acid	147.0	confidential	n/a
L-Histidine hydrochloride-H2O	210.0	confidential	n/a
L-Isoleucine	131.0	confidential	n/a
L-Leucine	131.0	confidential	n/a
L-Lysine hydrochloride	183.0	confidential	n/a
L-Methionine	149.0	confidential	n/a
L-Phenylalanine	165.0	confidential	n/a
L-Proline	115.0	confidential	n/a
L-Serine	105.0	confidential	n/a
L-Threonine	119.0	confidential	n/a
L-Tryptophan	204.0	confidential	n/a
L-Tyrosine	181.0	confidential	n/a
L-Valine	117.0	confidential	n/a
Vitamins
Choline chloride	140.0	confidential	n/a
D-Pantothenic Acid	238.0	confidential	n/a
Folic Acid	441.0	confidential	n/a
Myo-Inositol	180.0	confidential	n/a
Niacinamide	122.0	confidential	n/a
Pyridoxal hydrochloride	206.0	confidential	n/a
Riboflavin	376.0	confidential	n/a
Thiamine hydrochloride	337.0	confidential	n/a
Vitamin B12	1355.0	confidential	n/a
d-Biotin	244.0	confidential	n/a
Inorganic Salts
Ammonium Metavanadate NaVO3	117.0	confidential	n/a
Ammonium Molybdate ((NH4)6Mo7O24-4H2O)	1236.0	confidential	n/a
Calcium Chloride (CaCl2-2H2O)	147.0	confidential	n/a
Cupric sulfate (CuSO4-5H2O)	250.0	confidential	n/a
Ferric sulfate (FeSO4-7H2O)	278.0	confidential	n/a
Magnesium Chloride (MgCl2-6H2O)	203.0	confidential	n/a
Manganese Sulfate (MnSO4-H20)	169.0	confidential	n/a
Nickelous Chloride NiCl2 6H2O	238.0	confidential	n/a
Potassium Chloride (KCl)	75.0	confidential	n/a
Sodium Acetate (NaCH3COO.3H2O)	136.0	confidential	n/a
Sodium Bicarbonate (NaHCO3)	84.0	confidential	n/a
Sodium Chloride (NaCl)	58.0	confidential	n/a
Sodium Meta Silicate Na2SiO3 9H2O	284.0	confidential	n/a
Sodium Phosphate dibasic (Na2HPO4-7H2O)	268.0	confidential	n/a
Sodium Selenite (Na2SeO3)	173.0	confidential	n/a
Tin Chloride (SnCl2.2H2O)	226.0	confidential	n/a
Zinc sulfate (ZnSO4-7H2O)	288.0	confidential	n/a
Other Components
Adenine.HCl	172.0	confidential	n/a
D-Glucose (Dextrose)	180.0	confidential	n/a
DL-alpha-Lipoic Acid	206.0	confidential	n/a
Ethanolamine	61.0	confidential	n/a
HEPES	238.0	confidential	n/a
O-Phosphorylethanolamine	141.0	confidential	n/a
Phenol Red	376.4	confidential	n/a
Putrescine 2HCl	161.0	confidential	n/a
Sodium Pyruvate	110.0	confidential	n/a
Thymidine	242.0	confidential	n/a

Reference:

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