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Attune Flow Cytometer Models

여러분의 실험실에 적합한 Attune Flow Cytometer 모델을 찾아보세요

Attune Flow Cytometer 전 제품은 어쿠스틱 포커싱 기술을 활용한 컴팩트한 벤치탑형 세포 분석 장비로, 탁월한 고속 샘플 유속, 높은 막힘 저항성, 그리고 뛰어난 신뢰성과 같은 공통된 특징을 제공합니다. 아래 비교 가이드를 통해 Attune NxT Flow Cytometer, Attune CytPix Flow Cytometer, Attune Xenith Flow Cytometer를 비교하여 귀하의 연구 요구에 가장 적합한 모델을 선택하시기 바랍니다.
 

어떤 Attune 장비가 여러분께 적합할까요?


 
Attune Xenith Flow Cytometer

Attune CytPix Flow Cytometer

Attune NxT Flow Cytometer
제품 요약 UV 및 NIR 레이저를 포함한 고급 스펙트럴 기능을 제공하며, 기존 보상(compensation) 방식과 스펙트럴 언믹싱 분석을 모두 지원하는 Attune Flow Cytometer입니다. 고해상도 브라이트필드 카메라와 자동 이미지 분석 소프트웨어를 탑재하여, 표준 형광 및 산란 파라미터와 결합한 형태계측(morphometric) 분석을 지원하는 Attune Flow Cytometer입니다. 최대 14컬러/16파라미터 분석을 지원하는 고급형 고처리량 어쿠스틱 포커싱 벤치탑 플로우 사이토미터입니다.
적용 분야 Spectral high-parameter analyses
  • New users
  • Cell and gene therapy
  • Quality control
  • New users
  • High throughput, standard parameter analysis
  • Rare event detection
특징

Optics (fluorescence detection)

레이저 수

 6 (fixed) 2–4 (see table below) 1–4 (see table below)
검출 채널 수 57 channels with wavelength-tuned photomultiplier tubes (PMTs) (51 fluorescence channels); user-changeable, keyed filters 2 scatter channels, up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs); user-changeable, keyed filters 

Optical alignment

 Fixed alignment with pre-aligned welded fiber; no user maintenance required
Optics (imaging) Laser excitation N/A 405 nm laser with <50 nanosecond pulse width N/A
Pulse width N/A <50 ns N/A

Fluidics

 Acoustic focusing Yes Yes Yes
Custom sample flow rates Yes Yes Yes

Performance (fluorescence detection)

 Fluorescence sensitivity ≤30 MESF for FITC, ≤10 MESF for PE, ≤10 MESF for APC ≤80 MESF for FITC, ≤30 MESF for PE, ≤70 MESF for APC
Fluorescence resolution rCV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN) CV <3% for the singlet peak of propidium iodide-stained chicken erythrocyte nuclei (CEN)
Data acquisition rate

Up to 35,000 events/sec with all parameters
Maximum electronic speed >100,000 events/sec with all parameters 65,000 events/sec with all parameters

Performance (imaging)

 Image capture rate N/A Up to 6,000 images/sec depending on image size and event rate N/A
Objective N/A Magnification 20x, N/A
Numerical aperture 0.45
Pixel resolution N/A 0.3 microns/pixel N/A
Detection limit N/A Visually detect 800 nm particles N/A
Image analysis software Models N/A Thermo Fisher–supplied models: leukocytes, beads; User-trained models: based on sample or particle type N/A
Parameters/Features N/A 36 measured morphology parameters in 10 categories: shape features, intensity and texture features, object features, pixel features, particle interaction, particle count, similarity calculation, moment weighted, co-occurrence and system features  N/A
Sample size range of models N/A 5–20 µm N/A
Image processing speed N/A 1,000 images/second
(varies by image size and complexity)		 N/A
Spectral analysis software Spectral unmixing Spectral unmixing with autofluorescence subtraction and live unmixing, and use of tubes and wells N/A N/A

Quality and regulatory

 Instrument tracking Automated daily baseline and performance test with Levey-Jennings plots
Regulatory status For Research Use Only

Physical

 Dimensions (H x W x D) including fluid bottles ~50 x 86 x 61 cm (~19.5 x 34 x 24 in) ~49 x 58 x 43 cm (~19 x 23 x 17 in) ~40 x 58 x 43 cm (~16 x 23 x 17 in)
Biosafety hood compatibility Yes Yes Yes

Computer

 Software requirements Flowscape Invitrogen Attune Cytometric Software
Memory 64 GB 64 GB (4 x 16 GB) DDR4 2666 MHz UDIMM Non-ECC 32 GB
Hard drives 2 x 8 TB SSD, 560 MB/sec; controller RAID1, integrated 2 x 8 TB SSD, 2.5-inch Samsung 870 QVO, 560 MB/s 2 x 2 TB SATA 3.0 GB/s, 8MB data burst cache; Controller RAID1, integrated
Graphics processor NVIDIA RTX A2000 Nvidia Quadro P2200 N/A

Attune Nxt 와 CytPix 의 레이저 및 Detector 구성

Lasers Laser configuration Number of detection channels for included lasers Total detection channels* Attune CytPix Attune NxT
Violet, 405nm Blue, 488nm Yellow, 561nm Green, 532nm Red, 637nm
1 Blue
✝ 4 ✝ ✝ ✝ 6 N/A A24864
2 Blue/green
✝ 3 4 ✝ 9 N/A A28995
Blue/yellow
✝ 3 4 ✝ 9 A51842 A24861
Blue/red
✝ 4 ✝ ✝ 3 9 A51840 A24863
Blue/violet
4 4 ✝ ✝ ✝ 10 A51841 A24862
Blue/violet 6
6 3 ✝ ✝ 11 A51843 A29002
3 Blue/green/red
✝ 3 4 3 12 N/A A28997
Blue/red/yellow
✝ 3 4 3 12 A51845 A28993
Blue/green/violet
4 3 4 ✝ 13 N/A A28999
Blue/violet/yellow
4 3 4 ✝ 13 A51846 A24859
Blue/red/violet
4 4 ✝ ✝ 3 13 A51844 A24860
Blue/red/violet 6
6 3 ✝ 3 14 A51847 A29003
4 Blue/red/violet/green
4 3 4 3 16 N/A A29001
Blue/red/yellow/violet
4 3 4 3 16 A51848 A24858
Blue/red/yellow/violet 6
6 2 3 3 16 A51849 A29004

*Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel.

✝Laser upgrade available; green laser not available on Attune CytPix

Attune Nxt 와 CytPix 의 레이저 및 Detector 구성

Attune Xenith Flow Cytometer

Laser  Wavelength (nm) Beam-shaping optics (BSO)* (mW)
UV 349 54
Violet 405 100
Blue 488 50
Yellow 561 50
Red 637 90
NIR 781 90

* Amount of measured usable laser power after light has gone through the beam optics and shaping filters.

Attune NxT and Attune CytPix Flow Cytometers

Laser Wavelength (nm) Beam-shaping optics (BSO)* (mW) Diode power** (mW)
Violet 405 50 100
Blue 488 50 100
Green† 532 100 140
Yellow 561 50 100
Red 637 100 140

* Amount of measured usable laser power after light has gone through the beam optics and shaping filters.

** Vendor-specified theoretical maximum.

† Green laser not available on the Attune CytPix Flow Cytometer.

For Research Use Only. Not for use in diagnostic procedures.