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siRNA |
siRNA, or small interfering RNA, are double-stranded RNA molecules that play a role in the endogenous RNA interference (RNAi) pathway. siRNA functions by stopping gene expression through binding to specific mRNA molecules and facilitating their degradation via the RNA-induced silencing complex (RISC), preventing protein production. This regulation is sequence-specific, allowing precise control over gene expression. Utilizing siRNA is an effective method to knock down gene expression and study protein function across various cell types and organisms.
Selecting high-quality, validated siRNAs ensures effective and accurate gene silencing for research in gene function and regulation. We offer predesigned siRNA, backed by an industry leading guarantee, and custom synthetic siRNA solutions. All siRNAs are designed for optimal performance to help ensure robust and reproducible results.
| Silencer Select siRNA | Silencer siRNA | Stealth RNAi siRNA | |
|---|---|---|---|
| Features | Recommended: Higher knockdown, fewest off-targets | Cost-effective, guaranteed siRNA | Good knockdown, reduced off-targets |
| Potency (recommended working conc.) | 5 nM | 50 nM | 20 nM |
| Silencing guarantee | 2 of 2 siRNA | 2 of 3 siRNA | 2 of 3 siRNA |
| Target specificity | Highest | Moderate | High |
| Chemical modifications, length |
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| Gene types targeted |
| Protein-coding genes | Protein-coding genes |
| siRNA libraries |
| N/A | |
| Target species | Human, mouse, rat (for other species, order custom designs) | ||
| Custom siRNA ordering tool | Custom Silencer Select siRNA | Custom Silencer siRNA | Custom Stealth RNAi siRNA |
| Design and build | Deliver | Detect | Validate |
|---|---|---|---|
| Number of transfections from 5 nmol of siRNA | ||
|---|---|---|
| Plate type (Transfection volume) | 10 nM final siRNA concentration | 100 nM final siRNA concentration |
| 6 well (2.5 mL) | 200 | 20 |
| 12 well (1 mL) | 500 | 50 |
| 24 well (0.5 mL) | 100 | 10 |
| 96 well (0.1 mL) | 5,000 | 50 |
Explore available siRNA libraries to support your screening workflow.
Technical inquires:
Our Technical Application Scientists are available to help assist you at techsupport@thermofisher.com
Ordering & Order Status inquires:
If you have questions about pre-designed RNAi orders and order status, please contact us at genomicorders@thermofisher.com
If you have any questions about Custom RNAi orders and order status, please contact us at RNAiSupport@thermofisher.com
For Research Use Only. Not for use in diagnostic procedures.
