The iBind Flex Western Device is an automated western-processing device that performs every step from blocking to washes to antibody incubations via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source, shakers, or trays. Each solution is released from the iBind Flex device wells to an iBind Flex Card via SLF; the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction.
The iBind Flex western detection procedure minimizes hands-on time and is highly reproducible. Setup time is fast: within 2.5 h you are ready for the detection step. Compared to manual methods, the iBind Flex western system requires less primary antibody, enables reproducible results, and is compatible with downstream detection protocols.