iBind™ Flex Fluorescent Detection (FD) Solution Kit

La solución de detección fluorescente (FD) iBind Flex es compatible con la detección fluorescente posterior (por ejemplo, longitud de ondaMás información

Número de catálogo	Cantidad
SLF2019	1 kit

Número de catálogo SLF2019

Precio (MXN)

Cantidad:

1 kit

La solución de detección fluorescente (FD) iBind Flex es compatible con la detección fluorescente posterior (por ejemplo, longitud de onda dual infrarroja) y sirve como solución combinada de bloqueo, lavado y diluyente de anticuerpos para procesar hasta un midiblot, dos miniblots o seis tiras blot cortadas verticalmente cuando se usan con el dispositivo iBind Flex Western y las tarjetas iBind Flex. Cada kit contiene reactivos suficientes para 10 midiblots o 20 miniblots.

Más información sobre los sistemas iBind Western ›

Sistema iBind Flex Western
El sistema iBind Flex Western ofrece procesamiento automatizado de Western blotting y puede alojar varios formatos de blots, incluidas tiras midi, mini y cortadas verticalmente. Basado en la tecnología de flujo lateral secuencial (SLF), todos los pasos de bloqueo, incubación de anticuerpos y lavado durante la inmunodetección son automáticos. En comparación con los métodos manuales, el sistema iBind Flex Western requiere menos anticuerpo primario, permite obtener resultados reproducibles y es compatible con protocolos de detección posteriores.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Para utilizar con (equipo)Dispositivo iBind Flex Western

Contenido del kitTampón iBind™ Flex FD 5X, Aditivo 100X iBind™ Flex, iBind™ Flex FD SDS al 10 %

N.º de reacciones10 midiblots o 20 miniblots

Cantidad1 kit

Condiciones de envíoAprobado para su envío a temperatura ambiente o en hielo húmedo

Método de detecciónFluorescencia

Membrane CompatibilityPVDF, nitrocelulosa

Línea de productosiBind

Tipo de productoKit de solución flexible

Unit Size1 kit

Contenido y almacenamiento

• Tampón 5X iBind Flex FD
• Aditivo 100X iBind Flex
• SDS 10 % iBind Flex FD

Almacenar a 2–8 °C.

Preguntas frecuentes

Can I use conjugated primary antibodies with the iBind Flex Western Device?

Yes. When using a conjugated primary antibody and no secondary antibody, add the diluted primary antibody to well 1 and replace diluted secondary antibody (well 3) with 1X iBind Flex/iBind Flex FD Solution. Alternatively, for faster time to results, add the diluted primary antibody to well 1, leave wells 2 and 3 empty, and add 1X iBind Flex/ iBind Flex FD Solution to well 4. This procedure will save time (~60 min) but may result in higher background.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

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