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Protocols for Immunology
Immunology at Work Resource Center
Protocols
Accelerate your immunology research with protocols to help you plan and set up your experiments step by step.
Cell preparation
- Isolation of human peripheral blood mononuclear cells from whole blood
- Monocyte isolation from peripheral blood mononuclear cells
- NK cell isolation from peripheral blood mononuclear cells
- T cell isolation from peripheral blood mononuclear cells
- CD4+ T cell isolation from peripheral blood mononuclear cells
- CD8+ T cell isolation from peripheral blood mononuclear cells
- Regulatory T cell isolation from peripheral blood mononuclear cells
- T cell isolation from secondary lymphoid organs
- Immune cell counting using automated cell counters
- Mouse Bone Marrow Cell Isolation
- Mouse Spleen Cell Isolation Protocol
- Whole Blood Staining Protocol for Flow Cytometry Analysis
Immune cell stimulation
Sample preparation
Flow cytometry
- Staining cell surface targets for flow cytometry
- Staining intracellular antigens for flow cytometry
- Intracellular staining guide for human and mouse cytokines
- LIVE/DEAD fixable dead cell stains protocol
- Annexin V staining for flow cytometry
- Monitoring T cell proliferation with CellTrace Violet Cell Proliferation Kit
Immunoassays
For Research Use Only. Not for use in diagnostic procedures.