Technical Resources - Media Formulations

Neurobasal™-A Medium (1X) liquid

Neurobasal™ Media are basal media formulated to meet the neuronal cells special requirements.

They allow for long-term maintenance of the normal phenotype and growth of neuronal cells, and maintain pure populations of neuronal cells without the need of an astrocyte feeder layer. The concentration of NaCl is adjusted to bring the Osmolality to 260 +/- 10 mOsm

ComponentsMolecular WeightConcentration (mg/L)mM
Amino Acids
Glycine75.030.00.4
L-Alanine89.02.00.02247191
L-Arginine hydrochloride211.084.00.39810428
L-Asparagine-H2O150.00.830.0055333334
L-Cysteine121.031.50.2603306
L-Histidine hydrochloride-H2O210.042.00.2
L-Isoleucine131.0105.00.8015267
L-Leucine131.0105.00.8015267
L-Lysine hydrochloride183.0146.00.7978142
L-Methionine149.030.00.20134228
L-Phenylalanine165.066.00.4
L-Proline115.07.760.06747826
L-Serine105.042.00.4
L-Threonine119.095.00.79831934
L-Tryptophan204.016.00.078431375
L-Tyrosine181.072.00.39779004
L-Valine117.094.00.8034188
Vitamins
Choline chloride140.04.00.028571429
D-Calcium pantothenate477.04.00.008385744
Folic Acid441.04.00.009070295
Niacinamide122.04.00.032786883
Pyridoxal hydrochloride204.04.00.019607844
Riboflavin376.00.40.0010638298
Thiamine hydrochloride337.04.00.011869436
Vitamin B121355.00.00685.0184503E-6
i-Inositol180.07.20.04
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)111.0200.01.8018018
Ferric Nitrate (Fe(NO3)3"9H2O)404.00.12.4752476E-4
Magnesium Chloride (anhydrous)95.077.30.8136842
Potassium Chloride (KCl)75.0400.05.3333335
Sodium Bicarbonate (NaHCO3)84.02200.026.190475
Sodium Chloride (NaCl)58.04000.068.965515
Sodium Phosphate monobasic (NaH2PO4-H2O)138.0125.00.9057971
Zinc sulfate (ZnSO4-7H2O)288.00.1946.736111E-4
Other Components
D-Glucose (Dextrose)180.04500.025.0
HEPES238.02600.010.92437
Phenol Red376.48.10.021519661
Sodium Pyruvate110.025.00.22727273

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