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Neurobasal™-A Medium This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  10888022

Gibco™  Related applications: Mammalian Cell Culture | Primary Cell Culture | Stem Cell Culture

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Neurobasal®-A Medium is a basal medium formulated to meet the special cell culture requirements of post-natal and adult brain neuronal cells when used with GIBCO® B-27® Supplement. Neurobasal®-A Medium can be used to grow neuronal cells from hippocampus, cortex and other regions of the brain. Neurobasal®-A Medium allows for both long and short term maintenance of homogeneous populations of neuronal cells without the need of an astrocyte feeder layer.

Add Your Own Supplements
All Neurobasal® media contain no L-glutamine, L-glutamic acid, or aspartic acid. Neurobasal® media must be supplemented with 0.5 mM L-glutamine or GlutaMAX™-I Supplement and either a serum-free supplement (such as B-27® Supplement or N-2 Supplement), or with serum. For initial plating, you should add 25 µM glutamic acid.

Quality Control Testing
All Neurobasal® media are tested for pH, osmolality, endotoxin, and the absence of bacterial and fungal contamination. The osmolality of Neurobasal®-A was demonstrated to be optimal for postnatal and adult CNS neurons.

Neurobasal®-A Medium contains Phenol Red. If Phenol Red will interfere with your applications, we also provide Neurobasal®-A Medium Minus Phenol Red.

Neurobasal®-A Medium is optimized for post-natal and adult brain neurons. For growing pre-natal and embryonic neurons, we provide Neurobasal® Medium.

See the entire Neurobasal® product line.

Learn more about our neural cell culture products.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


Cell Type: Neuronal Cells (Post-Natal), Neuronal Tumor Cells
Amino Acids: No Aspartic Acid, No Glutamic Acid
Classification: Serum-Free
Culture Type: Feeder Cell-Free
Form: Liquid
Glutamine: No Glutamine
Phenol Red Indicator: Phenol Red
Product Size: 500 mL
Serum Level: Serum-Free
Green Features: Sustainable packaging
Shipping Condition: Room Temperature

Contents & storage

Store in refrigerator (2–8°C) and protect from light.


Manuals & protocols

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