More than 25 years ago, Dr. Greg Brewer designed and optimized B-27 neuronal culture supplement to improve survival of primary neurons in culture. Soon after, Gibco B-27 Supplement became the first commercially available serum-free neuronal cell culture supplement. The classic B-27 Supplement and nutritive variants have been widely adopted and successfully used in many other applications and cell types, including growth and maintenance of neural stem cells and PSC-derived neurons, 3D cell culture, and disease modeling.
B-27 supplement is a defined yet complex mixture of antioxidant enzymes, proteins, vitamins, and fatty acids that are combined in optimized ratios to support neuronal survival in culture. The original serum-free neuronal culture supplement formula developed by Dr. Gregory Brewer and colleagues is described in Brewer et al., J Neuroscience Res 35: 567-576, 1993 and Brewer and Cotman, Brain Res 494: 65-74, 1989 [1,2].
Thermo Fisher Scientific provides convenient, lot-tested B-27 supplements based on Dr. Brewer’s formulation to support neuroscience researchers around the globe. Variations on B-27 neuronal culture supplement include formulations without insulin and B-27 without Vitamin A.
Choose the relevant application below to locate and order the recommended B-27 supplement for your research.
|Maintenance/maturation of pre-natal/fetal primary neurons||B-27 Plus supplement||A3582801|
|Maintenance/maturation of post-natal and adult brain neurons||B-27 Plus supplement||A3582801|
|Proliferation of neural stem cells||B-27 supplement without vitamin A||12587010|
|Differentiation and maintenance/maturation of stem cell-derived neurons||B-27 supplement||17504044|
|Maintenance/maturation of stem cell-derived neurons||B-27 Plus supplement||A3582801|
|Studies of oxidative stress/damage, apoptosis, or where free radical damage to neurons occurs||B-27 supplement without AO (antioxidants)||10889038|
|Studies of insulin secretion or insulin receptors||B-27 supplement without insulin||A1895601|
|Electrophysiology studies||B-27 Plus Neuronal Culture System||A3653401|
Maintaining healthy long-term cultures (three weeks and beyond) of primary neurons can be challenging, as these cells are quite sensitive and tend to undergo progressive cell death during culture. B-27 Plus supplement promotes survival and maturation of primary neurons in culture.
Figure 1. Primary rat cortical neurons cultured in the B-27 Plus supplement for three weeks demonstrates extensive neurite networks (left) and high expression of synapsin (right).
The desire for more reliable and biologically relevant models has increased, so too has the necessity for a next-generation media system that can maintain and mature optimal densities of functional neurons over longer periods of time in vitro.
The next-generation Gibco B-27 Plus Neuronal Culture System, composed of B-27 Plus supplement and Neurobasal Plus Medium, improves upon the classic culture environment through raw material and manufacturing upgrades and minor formulation modifications. Together, these small changes yield big results in the maintenance and maturation of primary neurons to offer the following improved benefits:
Complex 3D models are becoming a favored system to study embryonic development and disease to more faithfully recapitulate in vivo neural architectures and physiology than traditional 2D cultures. A plethora of applications, spanning a wide range of cell types have benefited from B-27 neuronal culture supplements.
Figure 2. Neural stem cells (NSCs) produce spheroids in B-27 supplement. NSCs generated from Gibco Human Episomal iPSC Line, or H9 hESC, were cultured in maturation medium consisting of either B-27 supplement with Neurobasal Medium or in B-27 Plus Neuronal Culture System. Cells seeded in Gibco Nunclon Sphera 96-Well Microplates across a range of densities formed single neural spheroids within 24 hours.
Commercial Cell Therapy Manufacturing Gibco B-27 Supplement (serum free) has been the trusted standard for a variety of neuronal culture applications with citations in more than 11,000 publications. As research moves toward clinical applications, Gibco CTS B-27 Supplement XenoFree enables the translation from the bench to commercial manufacturing. This Gibco Cell Therapy Systems (CTS) product offers proven serum-free supplementation for the growth, expansion, differentiation, and maintenance of pluripotent stem cell (PSC)-derived cells.
Scientists are discovering that B-27 supplements can support three-dimensional cultures of neural and other cell types, enabling a wide variety of experiments using 3D cell cultures.
|Brain, PSCs||Modeling Parkinson’s disease in midbrain-like organoids||Smits et al. NPJ Parkinsons Dis. 2019;5;5 (1)1–8.|
|Modeling G2019S-LRRK2 sporadic Parkinson's disease in 3D midbrain organoids||Kim et al. Stem Cell Reports. 2019;12(3):518–531.|
|Breast||A CD146 FACS protocol enriches for luminal keratin 14/19 double positive human breast progenitors||Ísberg et al. Sci Rep. 2019; 9:14843.|
|Colon||Modeling the effect of prolonged ethanol exposure on global gene expression and chromatin accessibility in normal 3D colon organoids||Devall et al. PLoS ONE. 2020;15(1):e0227116.|
|Esophagus||Esophageal 3D organoids of MPV17-/-mouse model of mitochondrial DNA depletion show epithelial cell plasticity and telomere attrition||Guha et al. Oncotarget. 2019;10(58):6245–6259.|
|Fallopian tube||Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation||Kessler et al. Nat Commun. 2019; 0(1):1–4.|
|Heart||Generation of Heart Organoids Modeling Early Human Cardiac Development Under Defined Conditions||Israeli et al. (2020), BioRxiv 10.1101/2020.06.25.171611|
|Intestine||Extracellular vesicle release from intestinal organoids is modulated by Apc mutation and other colorectal cancer progression factors||Szvicsek et al. Cell Mol Life Sci. 2019; 76:2463–2476.|
|Kidney||Stem cell-derived kidney organoids: engineering the vasculature||Koning et al. Cell Mol Life Sci. 2020;77(12):2257–73.|
|Organoids as a new model for improving regenerative medicine and cancer personalized therapy in renal diseases||Grassi et al. Cell Death Dis. 2019; 10(3):1–5.|
|Lung||Modeling of fibrotic lung disease using 3D organoids derived from human pluripotent stem cells||Strikoudis et al. Cell Rep. 2019;27(12):3709–3723.|
|Prostate||Prostate organoid cultures as tools to translate genotypes and mutational profiles to pharmacological responses||Pappas et al. J Vis Exp. 2019;152:e60346|
|Retina||Transcriptome-based molecular staging of human stem cell-derived retinal organoids uncovers accelerated photoreceptor differentiation by 9-cis retinal||Kaya et al. Mol Vis. 2019; 25:663–678.|
|Sweat glands||Sweat gland organoids contribute to cutaneous wound healing and sweat gland regeneration||Diao et al. Cell Death Dis. 2019;10:238.|
For Research Use Only. Not for use in diagnostic procedures.