For nearly 20 years, researchers have been publishing their experiments using Gibco B-27 cell culture supplements. The first commercially available, serum-free supplement for neural cell culture, Gibco B-27 Serum Free Supplement was developed for the long-term culture of rat hippocampal and cortical neurons. Since its introduction, the publication history of B-27 supplements demonstrates that researchers are expanding the use of these supplements far beyond their original applications.
B-27 Supplements
The most-referenced neural cell culture supplement just got better
The desire for more reliable and biologically relevant models has increased, so too has the necessity for a next-generation media system that can maintain and mature optimal densities of functional neurons over longer periods of time in vitro. The new B-27 Plus Supplement is the next generation of the most-cited neuronal cell culture product, B-27 Supplement.
NEW The Gibco B-27 Plus Neuronal Culture System features an optimized formulation, upgraded manufacturing process, and more stringent quality control for raw materials and final product.
These improvements enable:
- Increased neuronal survival by more than 50%—the highest in the industry
- Accelerated neurite outgrowth
- Improved electrophysiological activity and maturation of neurons
Which B-27 supplement is right for you?
To order the right B-27 supplement for you, choose the application/cell-type tab below and then click on the product name or catalog number to order.
All Gibco neural cell culture reagents are optimized to work together as a complete media system. Need a complementary media or cells? Browse our neural cell culture product guide.
Standard Applications / Primary Neurons
| Application | Product name | Cat. No. |
|---|---|---|
| Maintenance/maturation of prenatal/fetal primary neurons |
B-27 Plus Supplement | A3582801 |
Neural stem cells and progenitor cells
| Application | Product name | Cat. No. |
|---|---|---|
| Proliferation of neural stem cells | B-27 Supplement, minus vitamin A | 12587010 |
| Differentiation and maintenance/maturation of stem cell–derived neurons | B-27 Plus Supplement | A3582801 |
| B-27 Supplement | 17504044 | |
| Xeno-free proliferation of neural stem cells | CTS B-27 Supplement, XenoFree minus Vitamin A | A3353501 |
| Xeno-free differentiation of neural stem cells | CTS B-27 Supplement, XenoFree | A1486701 |
Specialty applications
| Application | Product name | Cat. No. |
|---|---|---|
| Studies where XenoFree components are required | CTS B-27 Supplement, XenoFree | A14867-01 |
| Studies of oxidative stress/damage, apoptosis, or where free radical damage to neurons occurs |
B-27 Supplement, minus AO (antioxidants) |
10889038 |
| Studies of insulin secretion or insulin receptors | B-27 Supplement, minus Insulin |
A1895601 |
| Electrophysiology studies | B-27 Plus Neuronal Culture System | A365401 |
Standard Applications / Primary Neurons
| Application | Product name | Cat. No. |
|---|---|---|
| Maintenance/maturation of prenatal/fetal primary neurons |
B-27 Plus Supplement | A3582801 |
Neural stem cells and progenitor cells
| Application | Product name | Cat. No. |
|---|---|---|
| Proliferation of neural stem cells | B-27 Supplement, minus vitamin A | 12587010 |
| Differentiation and maintenance/maturation of stem cell–derived neurons | B-27 Plus Supplement | A3582801 |
| B-27 Supplement | 17504044 | |
| Xeno-free proliferation of neural stem cells | CTS B-27 Supplement, XenoFree minus Vitamin A | A3353501 |
| Xeno-free differentiation of neural stem cells | CTS B-27 Supplement, XenoFree | A1486701 |
Specialty applications
| Application | Product name | Cat. No. |
|---|---|---|
| Studies where XenoFree components are required | CTS B-27 Supplement, XenoFree | A14867-01 |
| Studies of oxidative stress/damage, apoptosis, or where free radical damage to neurons occurs |
B-27 Supplement, minus AO (antioxidants) |
10889038 |
| Studies of insulin secretion or insulin receptors | B-27 Supplement, minus Insulin |
A1895601 |
| Electrophysiology studies | B-27 Plus Neuronal Culture System | A365401 |
Table 1. Cell types and disease models cited in publications using Gibco neural cell culture reagents
| Primary cells | Brainstem, cerebellar, cortical, dopaminergic, DRG, enteric, GABAergic, glutamatergic, hippocampal, motor, neural progenitor, pancreatic, parasympathetic, retinal, septal, striatal, substantia nigral, sympathetic ganglia |
|---|---|
| Stem cells | Induced pluripotent stem cell, neural precursor, neural stem cell, neuroepithelial, cardiomyocytes |
| Glial cells | Astrocyte, microglia, oligodendrocytes, radial glia, satellite, schwann |
| Disease models | Alzheimer’s disease, amyotropyic lateral sclerosis (ALS), brain tumor, cerebral palsy, epilepsy, glioma, Huntington’s disease, multiple sclerosis, Parkinson’s disease, schizophrenia, spinal cord injury, stroke |
Neural cell culture resources
For Research Use Only. Not for use in diagnostic procedures.