Increase cell survival by more than 50% with new Gibco™ B-27™ Plus Neuronal Culture System.

B-27™ Supplement (50X), minus antioxidants

Catalog number:  10889038

Gibco™  Related applications: Mammalian Cell Culture | Primary Cell Culture | Stem Cell Culture

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B-27® Supplement Minus AO is a customized B-27® Supplement without 5 antioxidants (vitamin E, vitamin E acetate, superoxide dismutase, catalase, and glutathione). Antioxidants serve as free radical scavengers, and their absence makes this formulation ideal for use in studies of oxidative damage to neurons.

B-27® Supplement Minus AO is provided as a 50X liquid and is intended to be used with Neurobasal® Medium or Neurobasal®-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.

B-27® Supplement is suitable for use in most neuronal cell applications. For specialized applications and depending on your experimental needs, we provide alternative formulations of B-27® Supplement.

Quality Control Testing
B-27® Supplement is tested in a growth assay utilizing primary rat (Sprague Dawley) embryonic hippocampal neurons, 18 day gestation. Additional standard evaluations are for the absence of bacterial and fungal contaminants. B-27® is also tested for endotoxin at a 1X concentration.
For Research Use Only. Not for use in diagnostic procedures.


Cell Type: Neuronal Cells
Classification: Serum-Free
Concentrated: 50 X
Other Additives: Insulin, No Antioxidant Cocktail, Vitamin A
Product Size: 10 mL
Purity or Quality Grade: Cell Culture Grade
Shipping Condition: Dry Ice

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.


Manuals & protocols

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