Increase cell survival by more than 50% with new Gibco™ B-27™ Plus Neuronal Culture System.

B-27™ Supplement (50X), minus vitamin A

Catalog number:  12587010

Gibco™  Related applications: Mammalian Cell Culture | Primary Cell Culture | Stem Cell Culture

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B-27® Supplement Minus Vitamin A is a customized B-27® Supplement without Vitamin A. Vitamin A (retinol) can be converted to retinoic acid, which can induce neuronal differentiation. The absence of vitamin A makes this formulation ideal for use in the culture of stem cells.

B-27® Supplement Minus Vitamin A is provided as a 50X liquid and is intended to be used with Neurobasal® Medium or Neurobasal®-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.

For other applications, we provide alternative formulations of B-27® Supplement.

Quality Control Testing
B-27® Supplement is tested in a growth assay utilizing primary rat (Sprague Dawley) embryonic hippocampal neurons, 18 day gestation. Additional standard evaluations are for the absence of bacterial and fungal contaminants. B-27® is also tested for endotoxin at a 1X concentration.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use, unless otherwise stated.

For Research Use Only. Not for use in diagnostic procedures.


Cell Type: Neural Cells
Classification: Serum-Free
Product Line: B-27™
Product Size: 10 mL
Tests Performed: Endotoxin, Performance, Sterility
Shipping Condition: Dry Ice

Contents & storage

Store in freezer (-5 to -20°C) and protect from light.


Manuals & protocols

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