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B-27® Supplement (50X), minus vitamin A

Catalog number: 12587010

Gibco™  Related applications: Mammalian Cell Culture | Primary Cell Culture | Stem Cell Culture

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Description

B-27® Supplement Minus Vitamin A is a customized B-27® Supplement without Vitamin A. Vitamin A (retinol) can be converted to retinoic acid, which can induce neuronal differentiation. The absence of vitamin A makes this formulation ideal for use in the culture of stem cells.

B-27® Supplement Minus Vitamin A is provided as a 50X liquid and is intended to be used with Neurobasal® Medium or Neurobasal®-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.

For other applications, we provide alternative formulations of B-27® Supplement.

Quality Control Testing
B-27® Supplement is tested in a growth assay utilizing primary rat (Sprague Dawley) embryonic hippocampal neurons, 18 day gestation. Additional standard evaluations are for the absence of bacterial and fungal contaminants. B-27® is also tested for endotoxin at a 1X concentration.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use, unless otherwise stated.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Neuronal Cells, Stem Cells (Neural)
Classification: Serum-Free
Product Size: 10 mL
Other Additives: Antioxidant Cocktail, No Vitamin A, Insulin
Purity or Quality Grade: Cell Culture Grade
Shipping Condition: Dry Ice

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

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