B-27™ Supplement (50X), minus vitamin A
B-27™ Supplement (50X), minus vitamin A
인용 및 참조 문헌 (2)
Gibco™

B-27™ Supplement (50X), minus vitamin A

가장 많이 인용되는 신경 세포 supplement인 B-27 Supplement Minus Vitamin A는 오리지널 B-27 Supplement에서 비타민 A(retinyl acetate)를 제외하고 제공되는 최적화된자세히 알아보기
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카탈로그 번호수량
1258701010mL
12587001100 mL
카탈로그 번호 12587010
제품 가격(KRW)
231,000
온라인 행사
Ends: 31-Dec-2025
256,000
할인액 25,000 (10%)
Each
카트에 추가하기
수량:
10mL
제품 가격(KRW)
231,000
온라인 행사
Ends: 31-Dec-2025
256,000
할인액 25,000 (10%)
Each
카트에 추가하기
가장 많이 인용되는 신경 세포 supplement인 B-27 Supplement Minus Vitamin A는 오리지널 B-27 Supplement에서 비타민 A(retinyl acetate)를 제외하고 제공되는 최적화된 제품입니다. B-27 Supplement Minus Vitamin A는 분화 유도 없이, 부유 배양 또는 부착 단일층 배양에서 neurospheres 형태로 신경 전구체 및 줄기 세포를 배양하는데 이상적인 무혈청 supplement입니다. B-27 Supplement Minus Vitamin A는 50X 액체로 제공되며, Astrocyte feeder 층 없이 뉴런 세포를 배양하기 위해 Neurobasal 배지 또는 Neurobasal-A 배지와 함께 사용할 수 있습니다.

B-27 supplement는 대부분의 신경 세포와 관련된 적용 분야에서 사용하기에 적합합니다. 다른 분야로 적용하고자 하는 경우를 위해, B-27 supplement 대체 조성 및 Neurobasal 배지를 제공하고 있습니다.

B-27 Supplement를 사용하는 확립된 프로토콜을 확인하시려면 Gibco 신경생물학 프로토콜 핸드북을 참조하십시오.

Gibco 신경생물학 제품을 사용한 논문은 Gibco CellCite에서 확인하실 수 있습니다.

연구용으로만 사용하십시오. 진단용으로는 사용할 수 없습니다.
사양
세포 유형신경 전구 세포
농도50X
배양 유형포유류 세포 배양
제품라인B-27
수량10mL
혈청 수준무혈청
유통 기한12개월
배송 조건드라이아이스
소스무혈청
테스트됨엔도톡신, 성능, 멸균성, 마이코플라즈마
분류무혈청
형태액체
제품 유형세포 배양 Supplement
멸균멸균
Unit SizeEach
구성 및 보관
차광하여 냉동보관(-5 ∼ -20°C)

자주 묻는 질문(FAQ)

Can B-27 supplement be filtered?

Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I thaw B-27 Supplements in a 37 degrees C water bath or incubator?

We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all B-27™ Supplement (50X), minus vitamin A FAQs

인용 및 참조 문헌 (2)

인용 및 참조 문헌
Abstract
Patient-derived glioblastoma organoids as real-time avatars for assessing responses to clinical CAR-T cell therapy
Authors:Meghan Logun, Xin Wang, Yusha Sun, Stephen J Bagley, Nannan Li, Arati Desai, Daniel Y Zhang, MacLean P Nasrallah, Emily Ling-Lin Pai, Bike Su Oner, Gabriela Plesa, Donald Siegel, Zev A Binder, Guo-Li Ming, Hongjun Song, Donald M O'Rourke
Journal:Cell Stem Cell
PubMed ID:39657679
Patient-derived tumor organoids have been leveraged for disease modeling and preclinical studies but rarely applied in real time to aid with interpretation of patient treatment responses in clinics. We recently demonstrated early efficacy signals in a first-in-human, phase 1 study of dual-targeting chimeric antigen receptor (CAR)-T cells (EGFR-IL13Rα2 CAR-T cells) ... More
Type I astrocytes and microglia induce a cytokine response in an encephalitic murine coronavirus infection.
Authors:Lavi E, Cong L
Journal:Exp Mol Pathol
PubMed ID:32454103
'The pathogenesis of viral infections involves an immune response by cytokines, causing a deleterious effect on organ function, in addition to tissue destruction due to viral replication. Clinical symptoms and laboratory findings of the human coronavirus disease COVID-19, caused by the novel coronavirus SARS CoV-2, indicate cytokine involvement. Our laboratory ... More