Neurobasal® Medium This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 21103049

Gibco™  Related applications: Mammalian Cell Culture | Primary Cell Culture | Stem Cell Culture

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Neurobasal® Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used with GIBCO® B-27® Supplement. Neurobasal® Medium can be used to grow neuronal cells from hippocampus, cortex and other regions of the brain. Neurobasal® Medium allows for both long and short term maintenance of homogeneous populations of neuronal cells without the need of an astrocyte feeder layer.

Add Your Own Supplements
All Neurobasal® media contain no L-glutamine, L-glutamic acid, or aspartic acid. Neurobasal® media must be supplemented with 0.5 mM L-glutamine or GlutaMAX™-I Supplement and either a serum-free supplement (such as B-27® Supplement or N-2 Supplement), or with serum. For initial plating, you should add 25 µM glutamic acid.

Quality Control Testing
All Neurobasal® media are tested for pH, osmolality, endotoxin, and the absence of bacterial and fungal contamination.

Neurobasal® Medium contains Phenol Red. If Phenol Red will interfere with your applications, we also provide Neurobasal® Medium Minus Phenol Red.

Neurobasal® Medium is optimized for pre-natal and embryonic neurons. For growing post-natal and adult brain neurons, we provide Neurobasal®-A Medium.

See the entire Neurobasal® product line.

Learn more about neural cell culture products from Invitrogen at

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


Cell Type: Stem Cells (Neural), Neuronal Cells (Pre-Natal)
Form: Liquid
Glutamine: No Glutamine
Amino Acids: No Aspartic Acid, No Glutamic Acid
Serum Level: Serum-Free
Culture Type: Feeder Cell-Free
Product Size: 500 mL
Classification: Serum-Free
Phenol Red Indicator: Phenol Red
Green Features: Fewer resources used, Less waste
Shipping Condition: Room Temperature

Contents & storage

Store in refrigerator (2–8°C) and protect from light.


Manuals & protocols

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