Search
Get started with cryo-electron microscopy
Cryo-electron microscopy (cryo-EM) is a collection of analytical techniques that uses cryogenic freezing to characterize the structure of biological samples in their near-native, hydrated state. Advances in automation, integrated workflows, training, and broader access to cryo-EM facilities have helped lower the barrier to adoption, making these techniques more accessible to researchers across structural biology.
Among these approaches, single particle analysis (SPA) has become a widely used technique for studying proteins and macromolecular complexes. Whether you're transitioning from another structural biology technique or approaching structural characterization for the first time, this guide provides a practical introduction to SPA and the key considerations for getting started.
In this eBook, you will:
Learn the fundamentals of cryo-electron microscopy and single particle analysis
Compare SPA with other structural biology techniques
Determine whether SPA is the right technique for your research
Debunk common misconceptions about cryo-EM and SPA
Explore practical pathways for getting started with cryo-EM
Discover how SPA is being applied across the life sciences
