Titer (Affinity) Analysis

Affinity chromatography is based on the bispecific interaction between a biomolecule and its ligand. This interaction is reversible, making it possible to isolate specific compounds from complex matrices, such as cell culture used for monoclonal antibody (mAb) production. This approach is used to determine the concentration, or titer, of a biotherapeutic protein during the manufacturing process. Affinity titer is an extremely important technique in the biopharmaceutical industry, being used during several stages of the biotechnological protein production process.

Run fast, accurate titer analysis of mAbs in harvest cell cultures using Thermo Scientific MAbPac Protein A LC columns. This column provides high-throughput and accurate analyses through a combination of low backpressure and high efficiency. Its unique format makes automation of loading, binding, elution, and collection using biocompatible systems quick and easy. A nonporous, polymeric resin with a hydrophobic divinylbenzene core and a hydrophilic surface optimized for affinity separation makes this column rugged and reproducible.

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Obtain new benchmarks in accuracy, precision, and sensitivity in a biocompatible UHPLC system with state-of-the-art quaternary solvent blending. The Thermo Scientific Vanquish Flex Quaternary UHPLC system shares all the values inherent to the Vanquish product line, including an innovative design, focus on uptime, robustness, and reliability. Multiple detection options as well as Thermo Scientific Viper Fingertight Fitting connections throughout the system give you the performance you need with the ease of use you want. The combination makes this system ideal for biopharmaceutical and pharmaceutical applications. 

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Thermo Scientific Chromeleon Chromatography Data System (CDS) software unifies workflows for chromatography and routine quantitative MS analysis. Quickly and easily process and report UV and MS data in one application. Run your analyses in an enterprise GxP compliant environment—from method creation to quantitation.