Immuno-oncology is the study of the immune system within a cancer environment. Significant strides to reduce tumor burden have been made in this field with the development of antibodies, biologics, and adoptive T cell therapies. Current immuno-oncology needs include generating new therapeutics for better efficacy and safety through finding new targets and improving drug design.

Thermo Fisher Scientific has developed a wide variety of research tools to help you with your therapeutics research and development endeavors. Learn what flow cytometric reagents and analysis offer in a number of immuno-oncology research areas, including investigations into the tumor microenvironment, immune checkpoint analysis, and CAR T characterization.

Active areas of immune-oncology research

Tumor microenvironments are composed of cancer, stromal, immune and other cell types

Immune checkpoints are cell pathways crucial in maintaining a normal immune response and protecting tissues from damage when the immune system is activated

Precision therapy involves genetically modifying patient’s own T cells to target antigens selectively expressed on cancer cells

Flow cytometry: a useful tool in immuno-oncology research

Combining reagents and instruments can generate more complete and complex information about the immune system’s role in cancer. Learn about immuno-oncology workflows designed to enable you to spend less time learning protocols and more time performing experiments.

Prepare single-cell suspensions from tumor, blood, and cultured samples. Eliminate tedious isolation and expansion steps by using innovative and high-quality reagents.

Highlights include:

  • State-of-the-art Dynabeads activation and expansion technology mimics in vivo T cell activation
  • Fixation kits are compatible with analysis of most cellular antigens
  • Proven protocols to prepare cells from both culture and in vivo samples

Learn more

Thermo Fisher Scientific—including the eBioscience product portfolio—offers over 10,000 primary antibodies for flow cytometry use. The wide range of fluorochrome-conjugated antibodies identifies human, mouse, rat, or non-human primate cell antigens and can be multiplexed with our other assays.

Highlights include:

  • Specifically developed, validated, and manufactured for flow cytometry applications
  • Large number of antibodies conjugated to popular dyes including, FITC, and APC, Alexa Fluor, eFluor, and the new Super Bright polymer dyes
  • Technical support and online tools to help build multicolor panels

Learn more at thermofisher.com/flowantibodies


The novel PrimeFlow assay methodology employs a proprietary fluorescent in situ hybridization (FISH) and branched DNA (bDNA) signal amplification technique that enables the detection of up to four RNA transcripts in a single cell and can be combined with standard flow cytometry antibody staining.

Highlights include:

  • Superior analysis of gene expression as cells change over time or in response to stimuli
  • High-throughput ready with optimized multi-well kits and probes for many RNA targets
  • Assay for any protein when no antibody is available

Learn more at thermofisher.com/primeflow

Proliferation measurements are typically made based on DNA synthesis or on cellular metabolism parameters. Assays can report cell health, genotoxicity, or inhibition of tumor cell growth during drug development. CellTrace assays and Click-iT EdU offer sensitive reagents to measure proliferation in many cell types.

Highlights include:

  • Quantitation of newly synthesized DNA
  • Detection without denaturation of DNA
  • Compatibility with sensitive R-PE tandems and fluorescent proteins
  • Fast detection, in as little as 60 minutes
  • An alternative to the cumbersome BrdU assay

Learn more


Understanding the mechanisms of cell death and survival can represent a critical aspect of toxicological profiling and drug discovery. Invitrogen reagents and assays are designed to effectively study changes in the plasma membrane, the mitochondria, caspase activity, and DNA fragmentation and chromatin condensation as a result of apoptosis.

Highlights include:

  • Many assays to detect different targets found in early-to-late events
  • Range of fluorescent options to be excited from multiple laser excitation sources
  • Assays are compatible and used in multiplexing experiments

Learn more


Dead cells can give false-positive results as they nonspecifically bind to many reagents. Removing dead cells is a critical step for obtaining accurate flow cytometry results. Invitrogen cell viability reagents minimize the number steps to measure the percentage of live and dead cells.

Highlights include:

  • A wide range of dyes spanning the spectrum and used in many fluorescence channels to fit into multicolor panels
  • Fixable and non-fixable options

Learn more

The Attune NxT Flow Cytometer is a high-performance instrument with features, capabilities and specifications that support the demands of researchers at the frontier of immuno-oncology research in the fight against cancer. 

Highlights include:

Learn more at thermofisher.com/attune

Prepare single-cell suspensions from tumor, blood, and cultured samples. Eliminate tedious isolation and expansion steps by using innovative and high-quality reagents.

Highlights include:

  • State-of-the-art Dynabeads activation and expansion technology mimics in vivo T cell activation
  • Fixation kits are compatible with analysis of most cellular antigens
  • Proven protocols to prepare cells from both culture and in vivo samples

Learn more

Thermo Fisher Scientific—including the eBioscience product portfolio—offers over 10,000 primary antibodies for flow cytometry use. The wide range of fluorochrome-conjugated antibodies identifies human, mouse, rat, or non-human primate cell antigens and can be multiplexed with our other assays.

Highlights include:

  • Specifically developed, validated, and manufactured for flow cytometry applications
  • Large number of antibodies conjugated to popular dyes including, FITC, and APC, Alexa Fluor, eFluor, and the new Super Bright polymer dyes
  • Technical support and online tools to help build multicolor panels

Learn more at thermofisher.com/flowantibodies


The novel PrimeFlow assay methodology employs a proprietary fluorescent in situ hybridization (FISH) and branched DNA (bDNA) signal amplification technique that enables the detection of up to four RNA transcripts in a single cell and can be combined with standard flow cytometry antibody staining.

Highlights include:

  • Superior analysis of gene expression as cells change over time or in response to stimuli
  • High-throughput ready with optimized multi-well kits and probes for many RNA targets
  • Assay for any protein when no antibody is available

Learn more at thermofisher.com/primeflow

Proliferation measurements are typically made based on DNA synthesis or on cellular metabolism parameters. Assays can report cell health, genotoxicity, or inhibition of tumor cell growth during drug development. CellTrace assays and Click-iT EdU offer sensitive reagents to measure proliferation in many cell types.

Highlights include:

  • Quantitation of newly synthesized DNA
  • Detection without denaturation of DNA
  • Compatibility with sensitive R-PE tandems and fluorescent proteins
  • Fast detection, in as little as 60 minutes
  • An alternative to the cumbersome BrdU assay

Learn more


Understanding the mechanisms of cell death and survival can represent a critical aspect of toxicological profiling and drug discovery. Invitrogen reagents and assays are designed to effectively study changes in the plasma membrane, the mitochondria, caspase activity, and DNA fragmentation and chromatin condensation as a result of apoptosis.

Highlights include:

  • Many assays to detect different targets found in early-to-late events
  • Range of fluorescent options to be excited from multiple laser excitation sources
  • Assays are compatible and used in multiplexing experiments

Learn more


Dead cells can give false-positive results as they nonspecifically bind to many reagents. Removing dead cells is a critical step for obtaining accurate flow cytometry results. Invitrogen cell viability reagents minimize the number steps to measure the percentage of live and dead cells.

Highlights include:

  • A wide range of dyes spanning the spectrum and used in many fluorescence channels to fit into multicolor panels
  • Fixable and non-fixable options

Learn more

The Attune NxT Flow Cytometer is a high-performance instrument with features, capabilities and specifications that support the demands of researchers at the frontier of immuno-oncology research in the fight against cancer. 

Highlights include:

Learn more at thermofisher.com/attune

Cancer research webinar

Presented by: Charles Prussak, Pharm D, PhD, UCSD Moores Cancer Center

Title: The Complex Pharmacology of T-Cell CARs

Broadcast Dates: Dec 12 & 13, 2017

Watch broadcast