Composite material used in dental fillings, analyzed with SEM and energy-dispersive X-ray spectroscopy. Understanding the dispersion of the different components within the matrix is critical for the proper application of the material.
Butterfly wing imaged in a Quattro ESEM under low accelerating voltage and vacuum (80 Pa), revealing surface details. Low-energy electrons probe the top surface rather than penetrating deeper within the sample.
Resin-embedded, heavy-metal-stained neuronal tissue imaged in high vacuum. Left) Low-magnification overview. Right) High-magnification regions of interest, showing contrast differences resulting from backscattered electrons.