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HLA NGS Assays

Next-Generation Sequencing (NGS) technology has revolutionized the field of Human Leukocyte Antigen (HLA) typing, which is critical for organ transplantation, disease association studies, and immune response research. NGS offers a powerful, precise, and efficient approach for high resolution HLA typing, improving the matching process in transplantation and enhancing our understanding of the genetic foundations of the immune system.

We offer a comprehensive portfolio that meets the diverse needs of the HLA genotyping market and our One Lambda NGS assays are designed for simplicity and flexibility across sequencing platforms.

 

Find HLA sequencing assays and kits by application area

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Time sensitive typing

 
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Routine solid organ typing/
Hematopoietic Stem Cell Transplantation (HSCT) typing

 
  • One Lambda™ HybriType™ NGS assay - a hybrid-capture-based NGS solution for Illumina platforms that delivers high-resolution HLA typing across 18 loci, and ABO. Its probe-based design optimizes read balance, minimizes allele dropout, and streamlines the workflow.

Time sensitive typing

Routine solid organ typing and Hematopoietic Stem Cell Transplantation (HSCT) typing

Sequencing HLA kit comparison

 

AllType Rapid Assays

AllType Assays

AllType FASTplex Assays

HybriType NGS Assays

Library prep method

 Amplicon (Long Range PCR)

Amplicon

Amplicon

Hybrid capture

Instrument compatibility
  • Oxford Nanopore Technologies MinIon
  • Ion Torrent Gene Studio S5 & S5+
  • Illumina MiSeq & MiSeq i100
  • Illumina MiniSeq
  • Illumina iSeq100
  • Ion Torrent Gene Studio S5 & S5+
  • Illumina MiSeq & MiSeq i100
  • Illumina MiniSeq
  • Illumina iSeq100
  • Illumina MiSeq & MiSeq i100
  • Illumina MiniSeq
  • Illumina iSeq100

Barcode type

Combinatorial dual index

Combinatorial dual index

Combinatorial dual index

Unique dual index
DNA fragmentation method N/A Physical shearing Enzymatic shearing Enzymatic shearing

Hands-on time

1.5 hours

3.5 hours

1.5 hours

2.5 hours

Turnaround time

Same day

3 days

Next day

Next day

Samples per run
  • 1 (single sample)
  • 6-24 (multi sample)
  • 8-96
  • 384
  • 8-96
  • 6-96

Loci

 11 loci: HLA-A, B, C, DRB1, DRB345, DQB1, DQA1, DPB1, and DPA1 11 loci: HLA-A, B, C, DRB1, DRB345, DQB1, DQA1, DPB1, and DPA1 11 loci: HLA-A, B, C, DRB1, DRB345, DQB1, DQA1, DPB1, and DPA1 18 loci: A, B, C, DRB1, DRB345, DQB1, DQA1, DPB1, DPA1, E, F, G, H, J, MICA, MICB 

Resolution
  • 2 fields
  • 3 fields
  • 3 fields
  • Class I: 3 field
  • Class II: 4 field

Application
  • Time-sensitive typing (Single Sample)
  • Routine typing (Multi-Sample)
  • Routine Solid Organ Typing
  • Bone Marrow Typing
  • Routine Solid Organ Typing
  • Bone Marrow Typing
  • Routine Solid Organ
  • Bone Marrow Typing
  • Copy Number Variation analysis

One Lambda™ HybriType™ NGS assay

A hybrid-capture-based NGS solution for Illumina platforms that delivers high-resolution HLA typing across 18 loci, and ABO. Its probe-based design optimizes read balance, minimizes allele dropout, and streamlines the workflow.

AllType Rapid Assay

An amplification kit, targeting eleven HLA loci for in a single multiplex polymerase chain reaction (PCR) that can be utilized to prepare libraries for long range sequencing.

TypeStream Visual NGS Analysis Software

A stand-alone NGS software solution that supports analysis of both single-read and paired-end sequencing data produced using the One Lambda AllType NGS Assays.

Product support and software files

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Access One Lambda software, product documentation, and support files                                                   

 

Not all products listed are CE marked in the EU or 510(k)-cleared in the US for in vitro diagnostic use. Availability of product in each country depends on local regulatory marketing authorization status. Not all products are available in all countries. Please consult your local sales representative for details.